Basic Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Feb 28, 2017; 23(8): 1353-1366
Published online Feb 28, 2017. doi: 10.3748/wjg.v23.i8.1353
Figure 1
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Figure 1 Representative macroscopic images of rat colonic mucosa in non-colitics (A), colitics (B), Cd-EtOHE at 31. 25 mg/kg (C), Cd-EtOHE at 62.50 mg/kg (D), Cd-EtOHE at 125 mg/kg (E), and Cd-EtOHE at 250 mg/kg (F).Cd: Combretum duarteanum; EtOHE: Ethanolic extract.
Figure 2
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Figure 2 Representative macroscopic images of rat colonic mucosa in non-colitics (A), colitics (B), Cd-HexP at 31. 25 mg/kg (C), Cd-HexP at 62.50 mg/kg (D), Cd-HexP at 125 mg/kg (E), and Cd-HexP at 250 mg/kg (F).Cd: Combretum duarteanum; HexP: Hexane phase.
Figure 3
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Figure 3 Representative macroscopic images of rat colonic mucosa in non-colitics (A), colitics (B), Cd-EtOHE at 125 mg/kg (C), and Cd-HexP at 62. 5 mg/kg (D).Cd: Combretum duarteanum; HexP: Hexane phase; EtOHE: Ethanolic extract.
Figure 4
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Figure 4 Representative histological appearance of rat colonic mucosa in the non-colitic group (A), colitic group (B), Cd-EtOHE (C), and Cd-HexP (D). Aumento magnification × 40 (*goblet cells; → ulceration region; 50 μm). Colonic tissue sections were stained with hematoxylin and eosin, and observed under light microscope (magnification × 40). Cd: Combretum duarteanum; HexP: Hexane phase; EtOHE: Ethanolic extract.
Figure 5
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Figure 5 Effect of acute administration of Cd-EtOHE at 125 mg/kg and Cd-HexP at 62. 5 mg/kg on myeloperoxidase activity (MPO, U/mg protein) (A), tumor necrosis factor-alpha (TNF-α, pg/mg protein) (B), interleukin-1β (IL-1β, pg/mg protein) (C), interleukin-10 (IL-10, pg/mg protein) (D) in trinitrobenzene sulfonic acid-induced colitis model with relapse in rats (TNBS, 10 mg/animal). Data are expressed as the mean ± SEM. aP < 0.05, bP < 0.01 and cP < 0.001 vs colitic group. dP < 0.05, eP < 0.01 and fP < 0.001 vs non-colitic group. Cd: Combretum duarteanum; HexP: Hexane phase; EtOHE: Ethanolic extract.
Figure 6
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Figure 6 Photomicrograph of colonic samples from rats submitted to trinitrobenzene sulfonic acid-induced colitis model with relapse in rats after 21 d of treatment. A: Non-colitic group; B: Colitic group; C: Cd-EtOHE at 125 mg/kg; D: Cd-HexP at 62.5 mg/kg. Immunohistochemical localization of Line I: COX-2; Line II: PCNA; Line III: SOD (→ COX-2, PCNA and SOD respectively). COX-2: Cyclooxygenase-2; PCNA: Proliferating cell nuclear antigen; SOD: Superoxide dismutase; Cd: Combretum duarteanum; HexP: Hexane phase; EtOHE: Ethanolic extract.