Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Dec 7, 2015; 21(45): 12800-12813
Published online Dec 7, 2015. doi: 10.3748/wjg.v21.i45.12800
Figure 1
Figure 1 Plasma tumor necrosis factor-α (A), interleukin-6 (B), lipopolysaccharide (C), and D-lactate levels (D). Results are presented as mean ± SE (n = 6). aP < 0.05, vs the HS group at the same time point. HS: Hemorrhagic shock; BTED: Biliary tract external drainage; TNF-α: Tumor necrosis factor-α; IL-6: Interleukin-6; LPS: Lipopolysaccharide.
Figure 2
Figure 2 Western blot analysis of expression of occludin (A and B) and claudin-1 (A and C) in the ileum. Results are presented as mean ± SE (n = 9). aP < 0.05 vs the HS group at the same time point. HS: Hemorrhagic shock; BTED: Biliary tract external drainage.
Figure 3
Figure 3 Immunohistochemical analysis of expression of occludin in the ileum. Sham group (A), HS group (B: 0.5 h after resuscitation; C: 1 h after resuscitation; D: 2 h after resuscitation; E: 4 h after resuscitation; F: 6 h after resuscitation) and HS + BTED group (G: 0.5 h after resuscitation; H: 1 h after resuscitation; I: 2 h after resuscitation; J: 4 h after resuscitation; K: 6 h after resuscitation). The expression levels of occludin in the ileum in the HS + BTED group were enhanced significantly compared with the HS group at 4 h and 6 h after resuscitation. HS: Hemorrhagic shock; BTED: Biliary tract external drainage.
Figure 4
Figure 4 Immunohistochemical analysis of expression of claudin-1 in the ileum. Sham group (A), HS group (B: 0.5 h after resuscitation; C: 1 h after resuscitation; D: 2 h after resuscitation; E: 4 h after resuscitation; F: 6 h after resuscitation) and HS + BTED group (G: 0.5 h after resuscitation; H: 1 h after resuscitation; I: 2 h after resuscitation; J: 4 h after resuscitation; K: 6 h after resuscitation). The expression levels of claudin-1 in the ileum in the HS + BTED group were enhanced significantly compared with the HS group at 6 h after resuscitation. HS: Hemorrhagic shock; BTED: Biliary tract external drainage.
Figure 5
Figure 5 Hematoxylin and eosin staining (A) and histopathologic scores (B) of the ileum. Sham group (a), HS group (b: 0.5 h after resuscitation; c: 1 h after resuscitation; d: 2 h after resuscitation; e: 4 h after resuscitation; f: 6 h after resuscitation) and HS + BTED group (g: 0.5 h after resuscitation; h: 1 h after resuscitation; i: 2 h after resuscitation; j: 4 h after resuscitation; k: 6 h after resuscitation). Histopathologic scores are presented as mean ± SE (n = 6). aP < 0.05 vs the HS group at the same time point. HS: Hemorrhagic shock; BTED: Biliary tract external drainage.