Effect of intra-arterial infusion with triolein emulsion on rabbit liver

Figure 2 Histologic findings in the portal area from a rabbit in group 1. A: Light microscopic finding shows no specific abnormality in the hepatocytes and sinusoids (H and E, × 200); B: Electron microscopic finding shows multiple fat vacuoles (arrows) within the vascular lumen. However, there is no evidence of interstitial edema. The integrity of the endothelium is well preserved.

Figure 3 Histologic findings in the portal and central area from a rabbit in group 2. A: Light microscopic finding shows no specific abnormality in the hepatic artery, hepatocytes, sinusoids or central hepatic veins (HE, × 200); B: Electron microscopic finding shows multiple fat vacuoles (arrows) within the vascular lumen. However, there is no evidence of interstitial edema. The integrity of the endothelium is well preserved.

Figure 1 Gross findings of the hepatic surface from a rabbit in group 1 after Evans blue staining. A: Anterior surface of the right lobe shows blue staining; B: Cut surface of the liver shows blue-stained hepatic vessels.

Figure 4 Confocal microscopic images of the central area of rabbit liver with transferase-mediated dUTP nick-end labeling staining. A: Representative group 1 liver specimen shows no evidence of hepatocyte apoptosis. The two small dots with green staining represent apoptotic blood cells within the sinusoid (× 400); B: Representative group 2 liver specimen shows no evidence of apoptosis in the hepatocytes; C: Representative group 3 liver specimen reveals no evidence of hepatocyte apoptosis. The highly fluorescent signals that highlight intensely stained scattered diffuse nuclei are white blood cells within the sinusoids (× 400).