Ovarian hormone modulates 5-hydroxytryptamine 3 receptors mRNA expression in rat colon with restraint stress-induced bowel dysfunction

Figure 1 A: 5-HT₃R mRNA expression in the colon of restraint stress rats. Lane 1: Markers, 100-bp ladder; lane 2: sham-operated group; lane 3: OVX group; lane 4: OVX+E₂+P group. B: 5-HT₃R mRNA expression in the colon of restraint stress rats. The bar shows the relative optical density of 5-HT₃R band obtained from different groups. Values were reported as the ratio of 5-HT₃R to β-actin signals and normalized by the corresponding RT-PCR products for β-actin, used as the internal control. PCR was carried out for 35 cycles. Amplified cDNA products were separated by 20 g/L agarose gel electrophoresis. ^bP = 0.000 vs OVX group; ^dP = 0.000 vs sham-operated group.