Detection of frameshift mutations of RIZ in gastric cancers with microsatellite instability

Figure 1 MSI analysis of 70 paired HGCs by DHPLC. Curves at the bottom in both Panels A and B represent normal DNA as indicated by the specification on the bottom right, and the rest curves represent tumor DNA in specified cases. Column: DNASep^TM; mobile phase: 0.1 mol/L TEAA (pH7.0); linear gradient: 30%-51% B in 7 min; flow rate: 0.9 mL/min; temperature: 80 °C; detection: UV at 260 nm. Panel A: DHPLC chromatograms of HGCs at BAT26; Panel B: DHPLC chromatograms of HGCs at BAT25.

Figure 2 Typical elution profiles of DHPLC analysis and sequencing. Panel A: DHPLC elution profiles for RIZ(A)₉ tract, column temperature: 56 °C, flow rate: 0.9 mL/min, mobile phase: 0.1 mol/L TEAA (pH7.0), linear gradient: 51%-60% B in 4.5 min, detected with UV at 260 nm; Panel B: Sequence tracings for the same samples. The upper panel is a normal control, and the lower is a frameshift mutation (1-bp deletion). The arrow indicates the mutant nucleotide; Panel C: DHPLC elution profiles for pro704 LOH, column temperature: 50 °C, flow rate: 0.75 mL/min, mobile phase: 0.1 mol/L TEAA (pH7.0), linear gradient: 49%-55% B in 7 min, detected with UV at 260 nm.