Pan KF, Lu YY, Liu WG, Zhang L, You WC. Detection of frameshift mutations of RIZ in gastric cancers with microsatellite instability. World J Gastroenterol 2004; 10(18): 2719-2722 [PMID: 15309726 DOI: 10.3748/wjg.v10.i18.2719]
Corresponding Author of This Article
Dr. Wei-Cheng You, Department of Cancer Epidemiology, Peking University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing 100034, China. weichengyou@yahoo.com
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Brief Reports
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World J Gastroenterol. Sep 15, 2004; 10(18): 2719-2722 Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2719
Detection of frameshift mutations of RIZ in gastric cancers with microsatellite instability
Kai-Feng Pan, You-Yong Lu, Wan-Guo Liu, Lian Zhang, Wei-Cheng You
Kai-Feng Pan, Lian Zhang, Wei-Cheng You, Department of Cancer Epidemiology, Peking University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing 100034, China
You-Yong Lu, Beijing Laboratory of Molecular Oncology, Peking University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing 100034, China
Wan-Guo Liu, Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic/Mayo Medical School, Rochester, MN 55905, USA
Author contributions: All authors contributed equally to the work.
Supported by the Major State Basic Research Development Program of the Ministry of Science and Technology of China, No. G1998051203, and Beijing Municipal Commission Foundation for Science and Technology, No. H020920030130
Correspondence to: Dr. Wei-Cheng You, Department of Cancer Epidemiology, Peking University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing 100034, China. weichengyou@yahoo.com
Telephone: +86-10-88141035 Fax: +86-10-66175832
Received: December 28, 2003 Revised: January 10, 2004 Accepted: February 18, 2004 Published online: September 15, 2004
Abstract
AIM: To study the frameshift mutations of the retinoblastoma protein-interacting zinc finger gene RIZ in gastric cancer with microsatellite instability, and to identify two coding polyadenosine tracts of RIZ.
METHODS: Frameshift mutations at (A)8 and (A)9 tracts of RIZ were detected in 70 human gastric cancer (HGC) specimens by DHPLC and DNA sequencing. Microsatellite instability (MSI) status was assessed by two mononucleotide markers, BAT26 and BAT25, by means of denaturing high-performance liquid chromatography (DHPLC).
RESULTS: In 70 HGC samples, 8 (11.4%) were found positive for instabilities at BAT26 and BAT25. In 7 of the 8 cases with instabilities at both BAT26 and BAT25 (MSI-H), 1 was unstable at BAT26 but stable at BAT25. Frameshift mutations were identified in 4 (57.1%) of the 7 samples with MSI-H in the (A)9 tract of RIZ without mutations in the (A)8 tract. In contrast, frameshift mutations were found in neither of the polyadenosine tracts in 63 samples of MSI-L or MSI stable tumors. Pro704 LOH detection in 4 cases with frameshift mutations did not find LOH in these cases.
CONCLUSION: Frameshift mutations of RIZ may play an important role in gastric cancers with MSI.
