Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 21, 2016; 22(47): 10325-10340
Published online Dec 21, 2016. doi: 10.3748/wjg.v22.i47.10325
Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma
Orsolya Galamb, Alexandra Kalmár, Barbara Kinga Barták, Árpád V Patai, Katalin Leiszter, Bálint Péterfia, Barnabás Wichmann, Gábor Valcz, Gábor Veres, Zsolt Tulassay, Béla Molnár
Orsolya Galamb, Barnabás Wichmann, Gábor Valcz, Zsolt Tulassay, Béla Molnár, Molecular Medicine Research Group, Hungarian Academy of Sciences, H-1088 Budapest, Hungary
Orsolya Galamb, Alexandra Kalmár, Barbara Kinga Barták, Árpád V Patai, Katalin Leiszter, Bálint Péterfia, 2nd Department of Internal Medicine, Semmelweis University, H-1088 Budapest, Hungary
Gábor Veres, 1st Department of Paediatrics, Semmelweis University, H-1083 Budapest, Hungary
Author contributions: Galamb O, Kalmár A, Péterfia B and Molnár B designed the study; Patai ÁV, Veres G and Molnár B collected the samples; Galamb O, Patai ÁV, Leiszter K, Valcz G and Veres G contributed to the collection of clinical data and histological analysis of the samples; Galamb O, Kalmár A, Barták BK and Patai ÁV performed the experiments; Galamb O, Kalmár A, Wichmann B and Valcz G analyzed the experimental data; Tulassay Z and Molnár B contributed to the design and critical review of the manuscript, obtained fundings; all authors were involved in writing the paper, made a critical revision of the manuscript for important intellectual content and had final approval of the submitted and published versions.
Supported by the National Research, Development and Innovation Office, No. KMR-12-1-2012-0216; and the Hungarian Scientific Research Fund, No. OTKA-K111743.
Institutional review board statement: All routine colonic biopsy samples from the patients were taken after informed consent and ethical permission was obtained for participation in the study.
Conflict-of-interest statement: The authors declare that no conflict of interest exists.
Data sharing statement: Additional data are available in a supplementary file.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: Orsolya Galamb, PhD, 2nd Department of Internal Medicine, Semmelweis University, Szentkirályi str 46, H-1088 Budapest, Hungary.
Telephone: +36-1-2660926 Fax: +36-1-2660816
Received: June 22, 2016
Peer-review started: June 24, 2016
First decision: August 22, 2016
Revised: September 20, 2016
Accepted: November 13, 2016
Article in press: November 13, 2016
Published online: December 21, 2016
Processing time: 180 Days and 3 Hours
Core Tip

Core tip: Several age-related DNA methylation alterations could be observed during colorectal cancer (CRC) formation and progression affecting the mRNA expression of certain CRC- and adenoma-related key control genes such as hypermethylated secreted frizzled related protein 1 (SFRP1), spectrin repeat containing nuclear envelope protein 1 and hypomethylated cell migration-inducing protein. For the first time significantly lower SFRP1 methylation levels were demonstrated in colonic tissue from children (under 18 years) compared to healthy adults. The main CRC-associated signal transduction pathways, such as WNT signaling and PI3K/Akt pathways are also influenced during aging.