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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 14, 2015; 21(6): 1765-1774
Published online Feb 14, 2015. doi: 10.3748/wjg.v21.i6.1765
Published online Feb 14, 2015. doi: 10.3748/wjg.v21.i6.1765
Sirtuin 1 in rat orthotopic liver transplantation: An IGL-1 preservation solution approach
Eirini Pantazi, Mohamed Amine Zaouali, Mohamed Bejaoui, Emma Folch-Puy, Joan Roselló-Catafau, Experimental Hepatic Ischemia, Reperfusion Unit, Institute of Biomedical Research of Barcelona, Barcelona, 08036 Catalonia, Spain
Mohamed Amine Zaouali, Hassen Ben Abdennebi, Faculty of Pharmacy, Molecular Biology and Anthropology Applied to Development and Health, 5000 Monastir, Tunisia
Ana Teresa Varela, Carlos Marques Palmeira, Department of Life Sciences and Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal
Anabela Pinto Rolo, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal
Author contributions: Pantazi E and Zaouali MA designed and performed the experimental work; Pantazi E, Zaouali MA, Bejaoui M and Folch-Puy E provided protocols and analysed data; Zaouali MA and Bejaoui M established the animal experimental model; Varela AT, Rolo AP and Palmeira CM determined NAD+, NAMPT levels; Ben Abdennebi H, Palmeira CM and Roselló-Catafau J contributed to the critical analyses of the data; Pantazi E, Zaouali MA, Folch-Puy E and Roselló-Catafau J coordinated the experiments and wrote the paper; all authors have read and approved the final manuscript.
Supported by Fondo de Investigaciones Sanitarias, No. FIS PI12/00519; and Eirini Pantazi is the recipient of a fellowship from AGAUR, No. 2012FI_B00382, Generalitat de Catalunya, Barcelona, Catalonia, Spain.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Joan Roselló-Catafau, Professor, Experimental Hepatic Ischemia, Reperfusion Unit, Institute of Biomedical Research of Barcelona, IIBB-CSIC, C/ Rosselló 161, 7th floor, 08036 Barcelona, Spain. jrcbam@iibb.csic.es
Telephone: +34-93-3638300 Fax: +34-93-3638301
Received: August 5, 2014
Peer-review started: August 6, 2014
First decision: October 14, 2014
Revised: October 25, 2014
Accepted: November 7, 2014
Article in press: November 11, 2014
Published online: February 14, 2015
Peer-review started: August 6, 2014
First decision: October 14, 2014
Revised: October 25, 2014
Accepted: November 7, 2014
Article in press: November 11, 2014
Published online: February 14, 2015
Core Tip
Core tip: Sirtuin 1 (SIRT1) has been implicated in pathways associated with ischemia-reperfusion injury (IRI), but its role in rat orthotopic liver transplantation has not yet been established. In our study, SIRT1 protein expression levels and activity increased when Institut Georges Lopez 1 (IGL-1) preservation solution was supplemented with trimetazidine, which was associated with less hepatic injury and mitochondrial damage. The increased deacetylation of FoxO1 by SIRT1 agreed with less oxidative stress and the activation of the autophagy pathway. These findings support the notion that SIRT1 up-regulation may be an effective strategy for reducing IRI and improving liver transplantation outcome.