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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 7, 2015; 21(5): 1510-1517
Published online Feb 7, 2015. doi: 10.3748/wjg.v21.i5.1510
Published online Feb 7, 2015. doi: 10.3748/wjg.v21.i5.1510
Crude extract of maggots: Antibacterial effects against Escherichia coli, underlying mechanisms, separation and purification
Quan-Sheng Ge, Hui-Min Zhang, Xia Liu, Shou-Yu Wang, De-Cheng Lv, Xu-Dong Li, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
Author contributions: Zhang HM designed the research, analyzed the data and wrote the paper; Ge QS performed the research and wrote the paper; Liu X performed the research and analyzed the data; Wang SY performed the research; Lv DC analyzed the data and wrote the paper; Li XD performed the research.
Supported by National Natural Science Foundation of China, No. 81270052.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Hui-Min Zhang, the First Affiliated Hospital of Dalian Medical University, No. 222 Zhongshan Road, Xiagang District, Dalian 116011, Liaoning Province, China. quansheng0618@163.com
Telephone: +86-411-83635963 Fax: +86-411-8363563.
Received: July 13, 2014
Peer-review started: July 13, 2014
First decision: August 15, 2014
Revised: October 10, 2014
Accepted: October 21, 2014
Article in press: October 21, 2014
Published online: February 7, 2015
Peer-review started: July 13, 2014
First decision: August 15, 2014
Revised: October 10, 2014
Accepted: October 21, 2014
Article in press: October 21, 2014
Published online: February 7, 2015
Core Tip
Core tip: Previous studies have found the antibacterial activity of the maggots, but studies on the underlying mechanisms are still lacking. In this study, we separated and purified the crude extract of maggots using DEAE-Sepharose ion exchange chromatography and Sephacryls-200HR gel filtration chromatography. Agarose gel electrophoresis and fluorescence probe technique were used to investigate the antibacterial mechanism of the crude extract of maggots. We found that the protein of maggots weighing around 15 KD had the antibacterial effect and the crude extracts played the antibacterial role by destroying the cell membrane of Escherichia coli and disturbing the DNA replication of plasmids.