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World J Gastroenterol. Dec 21, 2014; 20(47): 17883-17893
Published online Dec 21, 2014. doi: 10.3748/wjg.v20.i47.17883
Published online Dec 21, 2014. doi: 10.3748/wjg.v20.i47.17883
Differential gene expression profiling of gastric intraepithelial neoplasia and early-stage adenocarcinoma
Xue Xu, Gui-Jun Fei, Xi Wu, Fang Yao, Xing-Hua Lu, Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China
Xue Xu, Ning An, Graduate School of Peking Union Medical College and Chinese Academy of Medical Science, Beijing 100730, China
Lin Feng, Yu Liu, Ning An, Shu-Jun Cheng, State Key Laboratory of Molecular Oncology, Cancer Institute Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China
Wei-Xun Zhou, Yuan Li, Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China
Ying-Cai Ma, Department of Gastroenterology, Qinghai Provincial People’s Hospital, Xining 810007, Qinghai Province, China
Author contributions: Xu X carried out most of the experiments and participated in drafting the manuscript; Lu XH and Cheng SJ were leaders of the project, who conceived, initiated, and designed the study; Feng L contributed to the design of all experiments and revised the manuscript; Wu X, Yao F, Lu XH, Ma YC and Fei GJ collected all the patient samples and contributed to the design of the experiments; Fei GJ and Xu X collected patient clinical data; An N and Xu X contributed to the RNA preparation; Feng L performed array hybridization with assistance from Xu X and An N; Xu X participated in the bioinformatics analyses with the guidance of Feng L and Liu Y; Xu X performed the real time qPCR and immunohistochemical staining experiments and statistical analysis with assistance from Feng L and Liu Y; Zhou WX and Li Y carried out most of the pathological diagnosis and immunohistochemical evaluation; Lu XH and Cheng SJ supervised the project; all authors read and approved the final manuscript.
Supported by The specific grants of Public-Funded Projects in the Health Industry, Grant 200902002
Correspondence to: Xing-Hua Lu, Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Shuai Fu Yuan 1, Dongcheng District, Beijing 100730, China. lxhbj2000@aliyun.com
Telephone: +86-10-69155016 Fax: +86-10-69155016
Received: May 4, 2014
Revised: June 5, 2014
Accepted: June 14, 2014
Published online: December 21, 2014
Revised: June 5, 2014
Accepted: June 14, 2014
Published online: December 21, 2014
Core Tip
Core tip: This is the first study to perform a comprehensive detection of the gene expression profiling of gastric low-grade and high-grade intraepithelial neoplasia and early-stage adenocarcinoma. This study collected precise samples and reports a clear distinction of gene expression profiles between gastric low-grade and high-grade intraepithelial neoplasia, thus providing molecular evidence for their different clinical application. The characteristic upregulated genes during gastric early carcinogenesis were involved in metabolism and the immune response and nuclear factor-κB pathway, whose expression was validated in independent samples through real-time TaqMan® polymerase chain reaction and immunohistochemical staining.