Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 14, 2019; 25(30): 4213-4221
Published online Aug 14, 2019. doi: 10.3748/wjg.v25.i30.4213
Novel magnetic compression technique for establishment of a canine model of tracheoesophageal fistula
Yi Gao, Rong-Qian Wu, Yi Lv, Xiao-Peng Yan
Yi Gao, Yi Lv, Xiao-Peng Yan, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shannxi Province, China
Yi Gao, Rong-Qian Wu, Yi Lv, Xiao-Peng Yan, National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shannxi Province, China
Author contributions: Yan XP and Lv Y designed the study; Gao Y and Yan XP performed the research and acquired the data; Gao Y and Yan XP analyzed the data; Yan XP, Gao Y, and Wu RQ drafted the manuscript; Gao Y, Wu RQ, Lv Y, and Yan XP contributed significantly to the revision of the manuscript.
Supported by the National Natural Science Foundation of China, No. 81700545; the Natural Science Basic Research Plan of Shaanxi Province of China, No. 2017JQ8021; and the Fundamental Research Funds for the Central Universities, No. xjj2018jchz14.
Institutional review board statement: This study was approved by the Institutional Review Board of the First Affiliated Hospital of Xi’an Jiaotong University.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the First Affiliated Hospital of Xi’an Jiaotong University.
Conflict-of-interest statement: The authors declare no conflict of interests.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The ARRIVE Guidelines have been adopted.
Open-Access: This is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Xiao-Peng Yan, PhD, Doctor, Surgeon, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277, West Yanta Road, Xi’an 710061, Shannxi Province, China. yanxiaopeng99@163.com
Telephone: +86-29-82657541
Received: April 8, 2019
Peer-review started: April 8, 2019
First decision: May 30, 2019
Revised: July 4, 2019
Accepted: July 5, 2019
Article in press: July 5, 2019
Published online: August 14, 2019
ARTICLE HIGHLIGHTS
Research background

Tracheoesophageal fistula in children is a rare human disease worldwide. To date, there is no ideal surgical strategy for the disease. The main reason is due to the lack of ideal animal models to mimic this disease.

Research motivation

The magnetic compression technique has been used to prepare the animal model of digestive tract anastomosis or vascular anastomosis. Therefore, we tried to use this new technique to establish a novel model mimicking tracheoesophageal fistula in children, hoping to provide a new surgical strategy for this disease.

Research objectives

To establish a canine model of tracheoesophageal fistula using the magnetic compression technique.

Research methods

Using a magnetic device consisting of daughter and parent Nd-Fe-B permanent magnets, a tracheoesophageal fistula model was established in male beagles through placing the device in the cervical esophagus. After 4~6 days of operation, gastroscopy/bronchoscopy, esophageal angiography, and histological analysis were carried out to identify the model.

Research results

After the operation, the esophageal mucosa and pseudostratified ciliated columnar epithelium were absent at the site of the fistula in the beagle model of tracheoesophageal fistula. Mean operating time was 4.33 ± 1.11 min, and mean time for successful establishment of the tracheoesophageal fistula model was 4.67 ± 0.75 d.

Research conclusions

A simple, minimally invasive, and feasible model of tracheoesophageal fistula in canine was first established by using the novel magnetic compression technique.

Research perspectives

Although providing a suitable model similar to tracheoesophageal fistula in children, the feasibility, applicability, safety, and efficacy of new surgical treatment for tracheoesophageal fistula using the specific model need to be explored in future studies.