Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 28, 2019; 25(28): 3775-3786
Published online Jul 28, 2019. doi: 10.3748/wjg.v25.i28.3775
MiR-205 mediated APC regulation contributes to pancreatic cancer cell proliferation
Rui-Feng Qin, Jia Zhang, Hao-Ran Huo, Zeng-Jiang Yuan, Jia-Dong Xue
Rui-Feng Qin, Jia Zhang, Hao-Ran Huo, Zeng-Jiang Yuan, Jia-Dong Xue, Third Department of General Surgery, Handan Central Hospital, Handan 056000, Hebei Province, China
Author contributions: Qin RF designed the research; Qin RF, Zhang J, Huo HR, Yuan ZJ, and Xue JD performed the research; Qin RF and Zhang J analyzed the data; Qin RF wrote the paper.
Institutional review board statement: The study was reviewed and approved by the Handan Central Hospital Institutional Review Board.
Conflict-of-interest statement: The authors declare no conflict of interest.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Rui-Feng Qin, MSc, Associate Chief Physician, Third Department of General Surgery, Handan Central Hospital, No. 15, Zhonghua South Street, Handan 056000, Hebei Province, China. ruifengqin89@163.com
Telephone: +86-133-8300-0998 Fax: +86-310-2110011
Received: March 27, 2019
Peer-review started: March 27, 2019
First decision: May 16, 2019
Revised: June 7, 2019
Accepted: June 22, 2019
Article in press: June 23, 2019
Published online: July 28, 2019
ARTICLE HIGHLIGHTS
Research background

Pancreatic cancer is known as a deadly malignancy in the world, and a sufficient treatment for the disease has not been found yet. We aimed to explore the regulatory mechanisms of microRNAs (miRNAs) in pancreatic cancer.

Research motivation

As a group of non-coding RNAs, miRNAs were found to paly important roles in human disease. In this study, we aimed to explore the miRNAs involved in pancreatic cancer and potential regulatory mechanisms of miR-205, which may contribute to pancreatic cancer treatment.

Research objectives

MiRNA expression pattern in pancreatic cancer and potential regulatory mechanisms of miR-205 and adenomatous polyposis coli (APC) were analyzed. The findings may contribute to clinical care of pancreatic cancer.

Research methods

Microarray analysis was used to explore the genome-wide miRNA expression profile in pancreatic cancer. QRT-PCR and Western blot were performed to validate gene expression. Bioinformatics analysis was performed to predict target genes and their potential functions. Dual luciferase reporter assay was used to validate the binding of miR-205 and APC. Proliferation was evaluated by MTT and colony formation assays.

Research results

A large number of differentially expressed miRNAs were identified in pancreatic cancer. MiR-205 was significantly up-regulated while APC was down-regulated in pancreatic cancer. Dual luciferase reporter assay showed that APC is a validated target of miR-205. Moreover, miR-205 could promote cell proliferation in pancreatic cancer by targeting APC.

Research conclusions

This study, for the first time, revealed that miR-205 mediated APC regulation contributed to pancreatic cancer development. Microarray analysis was used to fully disclose the genome-wide miRNAs expression profile in pancreatic cancer. Proliferation experiment showed that miR-205 could promote cell proliferation in pancreatic cancer cells by targeting APC, which could be considered as novel prognostic biomarkers for future clinical care.

Research perspectives

The aberrantly expressed miRNAs identified in pancreatic cancer may provide valuable resources for future cancer research.