Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 28, 2019; 25(28): 3775-3786
Published online Jul 28, 2019. doi: 10.3748/wjg.v25.i28.3775
MiR-205 mediated APC regulation contributes to pancreatic cancer cell proliferation
Rui-Feng Qin, Jia Zhang, Hao-Ran Huo, Zeng-Jiang Yuan, Jia-Dong Xue
Rui-Feng Qin, Jia Zhang, Hao-Ran Huo, Zeng-Jiang Yuan, Jia-Dong Xue, Third Department of General Surgery, Handan Central Hospital, Handan 056000, Hebei Province, China
Author contributions: Qin RF designed the research; Qin RF, Zhang J, Huo HR, Yuan ZJ, and Xue JD performed the research; Qin RF and Zhang J analyzed the data; Qin RF wrote the paper.
Institutional review board statement: The study was reviewed and approved by the Handan Central Hospital Institutional Review Board.
Conflict-of-interest statement: The authors declare no conflict of interest.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Rui-Feng Qin, MSc, Associate Chief Physician, Third Department of General Surgery, Handan Central Hospital, No. 15, Zhonghua South Street, Handan 056000, Hebei Province, China. ruifengqin89@163.com
Telephone: +86-133-8300-0998 Fax: +86-310-2110011
Received: March 27, 2019
Peer-review started: March 27, 2019
First decision: May 16, 2019
Revised: June 7, 2019
Accepted: June 22, 2019
Article in press: June 23, 2019
Published online: July 28, 2019
Processing time: 123 Days and 9.5 Hours
Abstract
BACKGROUND

Pancreatic cancer is a deadly malignancy with aggressive properties. MicroRNAs (miRNAs) participate in the pathogenesis of a variety of diseases and molecular processes by targeting functional mRNAs. Nevertheless, the regulatory role of miRNAs in signaling pathways involved in pancreatic cancer remains largely unknown.

AIM

To explore the molecular regulation involved in pancreatic cancer and potential mechanisms of miR-205.

METHODS

Microarray analysis was performed to investigate the expression profile of miRNAs in pancreatic cancer. Expression of miR-205 was validated by qRT-PCR. Target prediction and functional enrichment analysis were employed to seek potential target genes of miR-205 and potential functions of these genes. The target binding of miR-205 and adenomatous polyposis coli (APC) was validated by luciferase reporter assay. APC protein expression in pancreatic cancer was validated by qRT-PCR and Western blot. Proliferation was evaluated by MTT and colony formation assays.

RESULTS

A large number of miRNAs with altered expression were identified in pancreatic cancer. MiR-205 was significantly up-regulated. APC was found to be a validated target of miR-205 and down-regulated in pancreatic cancer. Proliferation experiments showed that miR-205 could promote cell proliferation in pancreatic cancer by targeting APC.

CONCLUSION

The above findings suggested that miR-205 mediated APC regulation contributes to pancreatic cancer development, which could be considered as a novel prognostic biomarker for clinical care.

Keywords: Pancreatic cancer; Microarray; MiR-205; Adenomatous polyposis coli; Proliferation

Core tip: A large number of microRNAs with altered expression were identified in pancreatic cancer. MiR-205 was found to be significantly up-regulated in pancreatic cancer. Adenomatous polyposis coli (APC) was found to be a validated target of miR-205 and down-regulated in pancreatic cancer. Proliferation experiments showed that miR-205 could promote cell proliferation in pancreatic cancer by targeting APC.