Zhou XB, Guo M, Quan LP, Zhang W, Lu ZM, Wang QH, Ke Y, Xu NZ. Detection of human papillomavirus in Chinese esophageal squamous cell carcinoma and its adjacent normal epithelium. World J Gastroenterol 2003; 9(6): 1170-1173 [PMID: 12800217 DOI: 10.3748/wjg.v9.i6.1170]
Corresponding Author of This Article
Dr Ning-Zhi Xu, Laboratory of Cell and Molecular Biology, Cancer Institute & Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China. xningzhi@public.bta.net.cn
Article-Type of This Article
Esophageal Cancer
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World J Gastroenterol. Jun 15, 2003; 9(6): 1170-1173 Published online Jun 15, 2003. doi: 10.3748/wjg.v9.i6.1170
Detection of human papillomavirus in Chinese esophageal squamous cell carcinoma and its adjacent normal epithelium
Xiao-Bo Zhou, Mei Guo, Lan-Ping Quan, Wei Zhang, Zhe-Ming Lu, Quan-Hong Wang, Yang Ke, Ning-Zhi Xu
Xiao-Bo Zhou, Lan-Ping Quan, Wei Zhang, Ning-Zhi Xu, Laboratory of Cell and Molecular Biology, Cancer Institute & Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China
Mei Guo, Zhe-Ming Lu, Yang Ke, Laboratory of Genetics, Beijing Institute for Cancer Research, School of Oncology, Peking University, No. 1 Da Hong Luo Chang St, Beijing 100034, China
Quan-Hong Wang, Department of Pathology, the Third People's Hospital of Shanxi Province, Taiyuan 030013, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr Ning-Zhi Xu, Laboratory of Cell and Molecular Biology, Cancer Institute & Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China. xningzhi@public.bta.net.cn
Telephone: +86-10-67738220 Fax: +86-10-67767548
Dr Yang Ke, Laboratory of Genetics, Beijing Institute for Cancer Research, School of Oncology, Peking University, No. 1 Da Hong Luo Chang St, Beijing 100034, China. keyang@bjmu.edu.cn
Telephone: +86-10-62091204 Fax: +86-10-62015681
Received: December 22, 2002 Revised: March 1, 2003 Accepted: March 5, 2003 Published online: June 15, 2003
Abstract
AIM: To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China.
METHODS: Twenty-three esophageal squamous cell carcinoma samples and the distal normal epithelium from Shanxi Province, and 25 more esophageal squamous cell carcinoma samples from Anyang city, two areas with a high incidence of esophageal cancer in China, were detected for the existence of HPV-16 DNA by PCR, mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV-16 E6 gene.
RESULTS: There were approximately 64% (31/48) patients having HPV-16 DNA in tumor samples, among them nearly two-thirds (19/31) samples were detected with mRNA expression of HPV-16 E6. However, in the normal esophageal epithelium from cancer patients, the DNA and mRNA of HPV-16 were found with much less rate: 34.7% (8/23) and 26.1% (6/23) respectively. In addition, at protein level detected by IHC assay, 27.1% (13/48) tumor samples had virus oncoprotein E6 expression, while only one case of normal epithelium was found positive.
CONCLUSION: HPV infection, especially type 16, should be considered as a risk factor for esophageal malignancies in China.
