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Wen CY, Ito M, Wang H, Chen LD, Xu ZM, Matsuu M, Shichijo K, Nakayama T, Nakashima M, Sekine I. IL-11 up-regulates Tie-2 expression during the healing of gastric ulcers in rats. World J Gastroenterol 2003; 9(4): 788-790 [PMID: 12679933 DOI: 10.3748/wjg.v9.i4.788]
Chun-Yang Wen, Mutsumi Matsuu, Kazuko Shichijo, Toshiyuki Nakayama, Ichiro Sekine, Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Japan
Chun-Yang Wen, Long-Dian Chen, Zhao-Min Xu, Department of Digestive Disease, Nanjing Gu-Lou Hospital, Medical School of Nanjing University, Nanjing 210008, Jiangsu Province, China
Masahiro Ito, Department of Pathology, National Nagasaki Medical Center, Japan
Hui Wang, Department of Infectious Disease, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China
Masahiro Nakashima, Tissue and Histopathology Section Division of Scientific Data Registry, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Japan
Author contributions: All authors contributed equally to the work.
Correspondence to: Chun-Yang Wen, M.D., Ph.D., Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. cywen518@net.nagasaki-u.ac.jp
Telephone: +81-95-849-7107 Fax: +81-95-849-7108
Received: November 29, 2002 Revised: January 6, 2003 Accepted: January 13, 2003 Published online: April 15, 2003
Abstract
AIM: To investigate Tie-2 expression during the repair of acetic acid-induced gastric ulcers in rats treated with recombinant human IL-11 (rhIL-11) and in untreated control animals.
METHODS: Gastric ulcers were induced in male Wistar rats by applying acetic acid to the fundus of the stomach. RhIL-11 (100 µg/kg twice daily, subcutaneously) was administered from two days before ulcer induction and continued for five days after the induction. Control rats received bovine serum albumin. Gastric specimens were collected at 3 and 5 d after the induction of ulcer for immunohistochemical observation, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR).
RESULTS: Immunohistochemical and Western blot analysis demonstrated that Tie-2 expression was enhanced in the rhIL-11-treated rats compared with the control animals at both intervals.
CONCLUSION: These findings suggested that IL-11 could accelerate ulcer healing, in part, by up-regulating Tie-2 expression and promoting angiogenesis.
