Esophageal Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2003; 9(4): 650-654
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.650
Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous cell carcinoma via proteomics approaches
Li-Yong Zhang, Wan-Tao Ying, You-Sheng Mao, Hong-Zhi He, Yu Liu, Hui-Xin Wang, Fang Liu, Kun Wang, De-Chao Zhang, Ying Wang, Min Wu, Xiao-Hong Qian, Xiao-Hang Zhao
Li-Yong Zhang, Hong-Zhi He, Yu Liu, Hui-Xin Wang, Fang Liu, Ying Wang, Min Wu, Xiao-Hang Zhao, National Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, Beijing, China
You-Sheng Mao, De-Chao Zhang, Department of Pectoral Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, Beijing, China
Wan-Tao Ying, Xiao-Hong Qian, Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China
Kun Wang, Beijing Yanjing Hospital, Beijing 100037, China
Author contributions: All authors contributed equally to the work.
Supported by the Major State Basic Research Development Program of China, No.G19980512 and No.2001CB510201; the National Hi-Tech R & D Program of China, No.2001AA227091 and No. 2001AA233061; National Natural Science Foundation of China, No. 39990570, No.30171049, 30225045 and No.39990600
Correspondence to: Dr. Xiao-Hang Zhao, National Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, Beijing, China. zhaoxh@pubem.cicams.ac.cn
Telephone: +86-10-67709015 Fax: +86-10-67709015
Received: November 26, 2002
Revised: December 11, 2002
Accepted: December 18, 2002
Published online: April 15, 2003
Abstract

AIM: To identify the differentially secreted proteins or polypeptides associated with tumorigenesis of esophageal squamous cell carcinoma (ESCC) from serum and to find potential tumor secreted biomarkers.

METHODS: Proteins from human ESCC tissue and its matched adjacent normal tissue; pre-surgery and post-surgery serum; and pre-surgery and normal control serum were separated by two-dimensional electrophoresis (2-DE) to identify differentially expressed proteins. The silver-stained 2-DE were scanned with digital ImageScanner and analyzed with ImageMaster 2D Elite 3.10 software. A cluster of protein spots differentially expressed were selected and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One of the differentially expressed proteins, clusterin, was down-regulated in cancer tissue and pre-surgery serum, but it was reversed in post-surgery serum. The results were confirmed by semi-quantitative reverse-transcription (RT)-PCR and western blot.

RESULTS: Comparisons of the protein spots identified on the 2-DE maps from human matched sera showed that some proteins were differentially expressed, with most of them showing no differences in composition, shape or density. Being analyzed by MALDI-TOF-MS and database searching, clusterin was differentially expressed and down-regulated in both cancer tissue and pre-surgery serum compared with their counterparts. The results were also validated by RT-PCR and western blot.

CONCLUSION: The differentially expressed clusterin may play a key role during tumorigenesis of ESCC. The 2DE-MS based proteomic approach is one of the powerful tools for discovery of secreted markers from peripheral.

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