Zhu HQ, Wang Y, Hu RL, Ren B, Zhou Q, Jiang ZK, Gui SY. Distribution and expression of non-muscle myosin light chain kinase in rabbit livers. World J Gastroenterol 2003; 9(12): 2715-2719 [PMID: 14669320 DOI: 10.3748/wjg.v9.i12.2715]
Corresponding Author of This Article
Professor Yuan Wang, Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Provience, China. wangyuan@mail.hf.ah.cn
Article-Type of This Article
Basic Research
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Hua-Qing Zhu, Yuan Wang, Ruo-Lei Hu, Qing Zhou, Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Province, China
Hua-Qing Zhu, Yuan Wang, Ruo-Lei Hu, Qing Zhou, Shu-Yu Gui, Anhui Province Key Laboratory of Genomic Research, Hefei 230032, Anhui Province, China
Bin Ren, Department of Pathology, BIDMC and Harvard Medical School, 99 Brookline, MA 02215 , Boston, U S A
Shu-Yu Gui, Department of Respiratory Disease, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China
Zhi-Kui Jiang, 105 Hospital of PLA, Hefei 230031, Anhui Province, China
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation of China, No. 39870324, Grant for Excellent Young Teachers of Ministry of education of China, No.39870324, National Science Foundation of AnHui Province, No.9904312
Correspondence to: Professor Yuan Wang, Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Provience, China. wangyuan@mail.hf.ah.cn
Telephone: +86-551-5161140
Received: May 10, 2003 Revised: May 23, 2003 Accepted: June 2, 2003 Published online: December 15, 2003
Abstract
AIM: To study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers.
METHODS: Human nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques.
RESULTS: The polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes.
CONCLUSION: nmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.
