Clinical Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 15, 2003; 9(1): 179-183
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.179
Role of NF-κB in multiple organ dysfunction during acute obstructive cholangitis
Bin Tu, Jian-Ping Gong, Hu-Yi Feng, Chuan-Xin Wu, Yu-Jun Shi, Xu-Hong Li, Yong Peng, Chang-An Liu, Sheng-Wei Li
Bin Tu, Jian-Ping Gong, Hu-Yi Feng, Chuan-Xin Wu, Yu-Jun Shi, Xu-Hong Li, Yong Peng, Chang-An Liu, Sheng-Wei Li, Department of General Surgery, the Second College of Clinical Medicine & the Second Affiliated Hospital of Chongqing University of Medical Science, 74 Linjiang Road, Chongqing 400010, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.39970719, 30170919
Correspondence to: Dr Jian-Ping Gong, Department of General Surgery, the Second College of Clinical Medicine & the Second Affiliated Hospital of Chongqing University of Medical Science, 74 Linjiang Road, Chongqing 400010, China. gongjianping11@hotmail.com
Telephone: +86-23-63766701 Fax: +86-23-63822815
Received: June 24, 2002
Revised: July 16, 2002
Accepted: July 22, 2002
Published online: January 15, 2003
Abstract

AIM: To elucidate the role of NF-κB activation in the development of multiple organ dysfunction (MOD) during acute obstructive cholangitis (AOC) in rats.

METHODS: Forty-two Wistar rats were divided into three groups: the AOC group, the group of bile duct ligation (BDL group), and the sham operation group (SO group). All the animals in the three groups were killed in the 6th and 48th hour after operation. Morphological changes of vital organs were observed under light and electron microscopy. NF-κB activation was determined with Electrophoretic Mobility Shift Assay (EMSA). Arterial blood gas analyses and the serum levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine were performed. The concentrations of TNF-α and IL-6 in plasma were also measured.

RESULTS: The significant changes of histology and ultrastructure of vital organs were observed in AOC group. By contrast, in BDL group, all the features of organs damage were greatly reduced. Expression of NF-κB activation in various tissues increased in AOC group when compared to other two groups. At 6 h, the arterial pH in three groups was 7.52 ± 0.01, 7.46 ± 0.02, and 7.45 ± 0.02, and the blood pCO2 was 33.9 ± 0.95 mmHg, 38.1 ± 0.89 mmHg, 38.9 ± 0.94 mmHg, there was difference in three groups (P < 0.05). At 48 h, the blood pHvalues in three groups was 7.33 ± 0.07, 7.67 ± 0.04, and 7.46 ± 0.03, and blood HCO3- was 20.1 ± 1.29 mmol·L-1, 26.7 ± 1.45 mmol·L-1 and 27.4 ± 0.35 mmol·L-1, there was also difference in three groups (P < 0.05). In AOC group, Levels of LDH, ALT, BUN and creatinine were 16359.9 ± 2278.8 nkat·L-1, 5796.2 ± 941.9 nkat·L-1, 55.7 ± 15.3 mg/dl, and 0.72 ± 0.06 mg/dl, which were higher than in SO group (3739.1 ± 570.1 nkat·L-1, 288.4 ± 71.7 nkat·L-1, 12.5 ± 2.14 mg/dl, and 0.47 ± 0.03 mg/dl) (P < 0.05). Levels of plasma TNF-α and IL-6 in AOC at 48 h were 429 ± 56.62 ng·L-1 and 562 ± 57 ng·L-1, which increased greatly when compared to BDL group (139 ± 16 ng·L-1, 227 ± 43 ng·L-1) and SO group (74 ± 10 ng·L-1, 113 ± 19 ng·L-1) (P < 0.05).

CONCLUSION: The pathological damages and the NF-κB activation of many vital organs exised during AOC. These findings have an important implication for the role of NF-κB activation in MOD during AOC.

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