Basic Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 15, 2003; 9(1): 169-173
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.169
A new cytokine: the possible effect pathway of methionine enkephalin
Xin-Hua Liu, Dong-Ai Huang, Fei-Yi Yang, Yan-Sheng Hao, Guo-Guang Du, Ping-Feng Li, Gang Li
Xin-Hua Liu, Fei-Yi Yang, Yan-Sheng Hao, Guo-Guang Du, Ping-Feng Li, Gang Li, Department of Biochemistry and Molecular Biology, Health Science Center, Peking University, Beijing 100083, China
Dong-Ai Huang, Department of Biochemistry, Hainan Medical College, Haikou 571101, Hainan Province, China
Author contributions: All authors contributed equally to the work.
Supported by National Science Foundation of China, No. 30060091
Correspondence to: Dr. Gang Li and Ping-Feng Li, Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China. ligang55@263.net
Telephone: +86-10-62092454
Received: June 1, 2002
Revised: June 24, 2002
Accepted: July 6, 2002
Published online: January 15, 2003
Abstract

AIM: To investigate experimentally the effects of methionine enkephalin on signal transduction of mouse myeloma NS-1 cells.

METHODS: The antigen determinate of delta opioid receptor was designed in this lab and the polypeptide fragment of antigen determinate with 12 amino acids residues was synthesized. Monoclonal antibody against this peptide fragment was prepared. Proliferation of Mouse NS-1 cells treated with methionine enkephalin of 1 × 10-6 mol·L-1 was observed. The activities of protein kinase A (PKA) and protein kinase C (PKC) were measured and thereby the mechanism of effect of methionine enkephalin was postulated.

RESULTS: The results demonstrated that methionine enkephalin could enhance the proliferation of NS-1 cells and the effect of methionine enkephalin could be particularly blocked by monoclonal antibody. The activity of PKA was increased in both cytosol and cell membrane. With reference to PKC, the intracellular activity of PKC in NS-1 cells was elevated at 1 × 10-7 mol·L-1 and then declined gradually as the concentration of methionine enkephalin was raised. The effects of methionine enkephalin might be reversed by both naloxone and monoclonal antibody.

CONCLUSION: Coupled with the findings, it in-dicates that the signal transduction systems via PKA and PKC are involved in the effects of methionine enkephalin by binding with the traditional opioid receptors, and therefore resulting in different biological effects.

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