Gastric Cancer
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2002; 8(1): 54-59
Published online Feb 15, 2002. doi: 10.3748/wjg.v8.i1.54
Differential display of vincristine-resistance-related genes in gastric cancer SGC7901 cell
Xin Wang, Mei Lan, Yong-Quan Shi, Ju Lu, Yue -Xia Zhong, Han-Ping Wu, Hui-Hong Zai, Jie Ding, Kai-Cun Wu, Bo-Rong Pan, Jian-Ping Jin, Dai-Ming Fan
Xin Wang, Mei Lan, Yong-Quan Shi, Han-Ping Wu, Hui-Hong Zai, Jie Ding, Kai-Cun Wu, Dai-Ming Fan, Institute of Digestive disease, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Ju Lu, Department of Electronic Engineering, Tsinghua University, Beijing 100084, China
Yue-Xia Zhong, Emergency Department, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China
Bo-Rong Pan, Oncology Center of Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Jian-Ping Jin, Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, 44106-4970, Ohio, USA
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30030140, 39970901
Correspondence to: Prof. Dai-Ming Fan, Institute of Digestive disease, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China. Daimfan@pub.xao
Telephone: +86-29-3375221 Fax: +86-29-2539041
Received: April 21, 2001
Revised: July 16, 2001
Accepted: August 15, 2001
Published online: February 15, 2002
Abstract

AIM: To isolate and clone the vincristine-resistine-related genes in gastric cancer SGC7901 cell line and to clarify the multidrug-resistant molecular mechanism of gastric cancer cells.

METHODS: The modified differential-display polymerase chain reaction (DD-PCR) was used to examine the differences in the mRNA composition of Vincristine-resistant gastric cancer SGC 7901 cells (SGC7901/VCR), induced by vincristine sulfate versus SGC7901 cells. The differentially expressed cDNA fragments were confirmed by reverseNorthern analysis, sequencing, BLAST analysis and Northern bolt analysis.

RESULTS: DD-PCR identified that 54 cDNA fragments were preferentially expressed in SGC 7901/VCR cells. When these cDNA fragments were analyzed by reverse Northern blot, 20 were reproducibly expressed at a high level in SGC7901/VCR. Sequencing and BLAST analysis revealed that seven of the genes were known genes: ADP-ribosylation factor 4, Cytochrome oxidase subunit II, Ss-A/Ro ribonucleoprtein autoantigen 60kd subunit, ribosomal protein S13, galaectin-8 gene, oligophrenin 1 mRNA, ribosomal protein L23 mRNA; thirteen of the genes were unknown genes. The length and abundance of the four unknown genes mRNA were further confirmed by Northern blot analysis.

CONCLUSION: The twenty differential known and unknown genes may be related to the vincristine-resistant mechanism in human gastric cancer SGC7901 cell line.

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