Basic Research
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2002; 8(1): 135-138
Published online Feb 15, 2002. doi: 10.3748/wjg.v8.i1.135
Construction of HCV-core gene vector and its expression in cholangiocarcinoma
Xiao-Fang Liu, Sheng-Quan Zou, Fa-Zu Qiu
Xiao-Fang Liu, Sheng-Quan Zou, Fa-Zu Qiu, Department of General Surgery of Tongji Hospital, Wuhan 430030, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr.Xiao-Fang Liu, Department of Genneral Surgery of Tongji Hospital, 1095 Jiefang Road, Wuhan 430030, Hubei Province, China.
Telephone: +86-27-83662134
Received: April 21, 2001
Revised: August 6, 2001
Accepted: August 15, 2001
Published online: February 15, 2002

AIM: To establish an experimental model for exploring the role of hepatitis C virus (HCV) in the development of cholangiocarcinoma.

METHODS: Recombinant plasmid of HCV-core gene was constructed with molecular cloning technique and transfected into QBC939 cells with lipofection. After it was selected with G418, resistant colonies were obtained. The colonies were analysed by immunocytochemistry and Western blotting.The morphology was observed under transmission electron microscope(TEM) and microscope.

RESULTS: The recombinant plasmid was proved to carry the target gene by PCR and restriction enzymed mapping. Moreover, it could express HCV-C protein efficiently in QBC939 cells. The HCV-like particles were found in the cytoplasm by electron microscope, which were spherical with a diameter of 50 nm-80 nm possessing outer membrane.The transfected cells had lower differentiation and higher malignant degree under microscope.

CONCLUSION: Because HCV-core gene could express steadily in cholangiocarcinoma cells,the transfected tumor cells(QBC939-HCVC) could be used to study the effect of HCV in the development of cholangiocarcinoma.

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