Original Research
Copyright ©The Author(s) 2001. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 2001; 7(6): 864-867
Published online Dec 15, 2001. doi: 10.3748/wjg.v7.i6.864
Relationship between genotype and phenotype of flagellin C in Salmonella
Wan-Sheng Ji, Jia-Lu Hu, Jun-Wen Qiu, Bo-Rong Pan, Dao-Rong Peng, Bing-Long Shi, Shao-Juan Zhou, Kai-Chun Wu, Dai-Ming Fan
Wan-Sheng Ji, Jia-Lu Hu, Jun-Wen Qiu, Bo-Rong Pan, Bing-Long Shi, Shao-Juan Zhou, Kai-Chun Wu, Dai-Ming Fan, Chinese PLA Institute of Digestive Diseases, Department of Bacteriology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Dao-Rong Peng, Department of Bacteriology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Jia-Lu Hu, Chinese PLA Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. jiwsh@netease.com
Telephone: +86-29-3375229 (lab), Fax: +86-29-7432505 (home)
Received: June 19, 2001
Revised: July 9, 2001
Accepted: July 16, 2001
Published online: December 15, 2001
Abstract

AIM: To discover the relationship between the genotype and antigen serotype of flagellin C among Salmonella strains.

METHODS: Fragment of Salmonella flagellin C in plasmid pLS408 was cloned, sequenced and compared with the corresponding sequence in other strains. Salmonella strains including two typhi strains, one paratyphoid strain, one enteritidis and one typhimurium strain were isolated from outpatients. Genome DNA was purified respectively from these clinical isolates, then the corresponding flagellin C fragment was amplified by polymerase chain reaction, and the amplification products were analyzed by agarose gel electrophoresis.

RESULTS: The cloned fragment includes 582 nucleotides encoding the variable region and partial conservative region of Salmonella flagellin C in plasmid pLS408. With comparison to the corresponding sequences reported previously, there is only a little difference from other strains with the same flagellar serotype in both nucleotide and amino acid level. Specific PCR products were amplified in Salmonella strains with flagellar serotype H-1-d including S. muenchen, typhi and typhimurium, but not in S. paratyphoid C or S. enteritidis strains.

CONCLUSION: In this experiment, the specificity of nucleotide sequence could be found in flagellin C central variable regions as it exists in flagellar serotypes in Salmonella. It may be helpful to developing a rapid, sensitive, accurate and PCR-based method to detect Salmonella strains with serotype H-1-d.

Keywords: Salmonella; flagellin C; polymerase chain reaction; serotype; genotype