Sun HC, Li XM, Xue Q, Chen J, Gao DM, Tang ZY. Study of angiogenesis induced by metastatic and non-metastatic liver cancerby corneal micropocket model in nude mice. World J Gastroenterol 1999; 5(2): 116-118 [PMID: 11819406 DOI: 10.3748/wjg.v5.i2.116]
Corresponding Author of This Article
Dr. Hui-Chuan Sun, Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, #136 Yi Xue Yuan Road, Shanghai 200032, China. zytang@srcap.stc.sh.cn
Article-Type of This Article
Original Articles
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Hui-Chuan Sun, Xiao-Ming Li, Qiong Xue, Jun Chen, Dong-Mei Gao, Zhao-You Tang, Liver Cancer Institute and Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China
Hui-Chuan Sun, male, born on 1968-12-12 in Deyang City, Sichuan Province, Han Nationality, graduated from Shanghai Medical University in 1992. Now he is a Ph.D. graduate student in the Graduate School of Shanghai Medical University, studying the mechanisms of metastasis of liver cancer, especially in the field of angiogenesis. He has published 6 papers.
Author contributions: All authors contributed equally to the work.
Supported by the Fund for Leading Specialty of Shanghai Metropolitan Bureau of Public Health.
Correspondence to: Dr. Hui-Chuan Sun, Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, #136 Yi Xue Yuan Road, Shanghai 200032, China. zytang@srcap.stc.sh.cn
Telephone: +86-21-64037181 Fax: +86-21-64037181
Received: January 3, 1999 Revised: February 1, 1999 Accepted: February 12, 1999 Published online: April 15, 1999
Abstract
AIM To study the angiogenesis induced by liver cancer with different metastatic potentials using corneal micropocket model in nude mice.
METHODS Corneal micropockets were created in nude mice. Tumor tissues and liver tissues were implanted into the corneal micropockets. Angiogenesis was observed using a digital camera under slit-lamp biomicroscope, and compared among different grafts and incision alone. Vascular responses were recorded in regard to the range, number and length of new blood vessels toward the grafts or incisions.
RESULTS Vascular responses induced by tumor tissues were grea ter than those by incision alone and liver tissue grafts. LCI-D20 induced more intensive angiogenesis than LCI-D35.
CONCLUSION Highly metastatic liver cancer LCI D20 was more angiogenic than low metastatic cancer LCI D35 and liver tissue. Micropocket was a useful model to study dynamic process of angiogenesis in vivo.
