Published online Feb 15, 1999. doi: 10.3748/wjg.v5.i1.41
Revised: June 20, 1998
Accepted: October 9, 1998
Published online: February 15, 1999
AIM To investigate the protective effects of polydatin (PD) against injury to primarily cultured rat hepatocytes induced by CCl4.
METHODS Rat hepatocytes were separated by methods of liver infusion in vivo and cultured medium (7.5 × 105 cells/mL). Two mL or 0.2 mL was added into 24-well or 96-well plates respectively. Twenty-four hours after cell preculture, PD at concentrations of 10-7 mol/L-10-4 mol/L was added into each plate. At the same time injury to hepatocytes was induced by adding 10 mmol/L-CCl4. Then, 0.1 mL or 1 mL-culture solution was removed from the 96-well or 24-well plates at 6 h, 12 h, 24 h and 48 h after CCl4 intox-ication respectively for the determination of GPT, GSH and MDA. At 48 h, the survivability of rat hepatocytes was assayed by the MTT colormetric method.
RESULTS After CCl4 challenge, the release of GPT and the form ation of MDA in rat hepatocytes markedly increased and maintained at a high level in 48 h, whereas PD with different concentrations could markedly inhibit this elevation with 10-5 mol/L PD having the strongest effects and inhibiting rate was over 50%. PD could also im-prove the decreased content of GSH caused by CCl4 in accordance with the doses used. CCl4 ev-idently decreased the he patocyte survivability from 91.0% ± 7.9% to 35.4% ± 3.8%. On the other hand, PD at 10-7 mol/L-10-4 mol/L could reverse this change and improve t he cell survival rates to 56.1% ± 5.2%, 65.8% ± 5.0%, 88.7% ± 6.8% and 75.2% ± 7.3%, respectively.
CONCLUSION PD at 10-7 mol/L-10-4 mol/L could protect primarily cultured rat hepatocytes against CCl4 induced injury.