Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 15, 1998; 4(3): 219-221
Published online Jun 15, 1998. doi: 10.3748/wjg.v4.i3.219
Relationship between expression of laminin and pathological features in human colorectal carcinoma
Shu Feng, Yu-Ying Wang, Jin-Dan Song
Shu Feng, Yu-Ying Wang, Microbial Engineering Department, Institute of Applied Ecology, Academia Sinica, Shenyang 110015, Liaoning Province, China
Jin-Dan Song, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, Liaoning Province, China
Shu Feng, female, born on 1965-02-09, graduated from Jiamusi Medical College in 1986, got a master degree in 1989 and doctorate degree in 1996 in China Medical University, and now working as a postdoctorate in the Institute of Applied Ecology, Academic Sinica, having had 13 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Shu Feng, Microbial Engineering Department, Institute of Applied Ecology, Academia Sinica, Shenyang 110015, Liaoning Province, China
Telephone: +86-24-3916243
Received: November 26, 1997
Revised: January 10, 1998
Accepted: February 21, 1998
Published online: June 15, 1998

AIM: To study the expression and significance of laminin in human colorectal carcinoma.

METHODS: Using the monoclonal antibody to laminin and streptavidin-peroxidase immunohistochemical method, the expression of laminin in 63 cases of human colorectal carcinoma tissues was determined.

RESULTS: In normal marge intestinal mucosa adjacent to carcinoma, laminin was largely restricted to basement membrane in continuous linear pattern. In contrast, human colorectal carcinomas exhibited a progressive loss of an intact basement membrane that was correlated with decreasing differentiation degree. Well and moderately differentiated tumors exhibited a thin basement membrane with intermittent disruptions, and poorly differentiated tumors exhibited no areas of intact basement membrane. An association was found between lack of basement membrane laminin immunohistochemical staining in colorectal carcinoma and poorly differentiated tumor (P < 0.01).

CONCLUSION: Immunohistochemical staining for laminin could provide a very useful indexfor the determination of the differentiation degree of colorectal carcinoma.

Keywords: rectal neoplasms/pathology, colonic neoplasms/pathology, laminin/metabolism, immunohistochemistry