Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 1998; 4(2): 144-146
Published online Apr 15, 1998. doi: 10.3748/wjg.v4.i2.144
Experimental research on production and uptake sites of TNFα in rats with acute hemorrhagic necrotic pancreatitis
Ren-Yi Qin, Sheng-Quan Zou, Zai-De Wu, Fa-Zu Qiu
Ren-Yi Qin, Sheng-Quan Zou, Zai-De Wu, Fa-Zu Qiu, Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan 430030, Hubei Province, China
Ren-Yi Qin, male, born on 1963-04-10 in Zhenyi City, Guizhou Province, graduated from Guiyang Medical College with the bachelor degree in 1986, and from Zhejiang Medical University with M.D. in 1995. Now associate professor and having 21 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Ren-Yi Qin, Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan 430030, Hubei Province, China. ryqin@tjh. tjmu. edu. cn.
Telephone: +86·27·363881 ext 3412
Received: December 5, 1997
Revised: December 30, 1997
Accepted: January 10, 1998
Published online: April 15, 1998

AIM: To determine the site of production and uptake of tumor necrotic factor alpha (TNFα), and evaluate the relationship between serum TNFα and plasma endotoxin (ET) in rats with acute hemor-rhagic necrotic pancreatitis (AHNP).

METHODS: Sprague Dawley rats were divided into AHNP group and control group (n = 12). AHNP model was induced by retrograde injection of 5% sodium taurocholate via pancreatic bile duct. The blood samples were obtained through portal vein 2 and 6 h after the operation.

RESULTS: The contents of TNFα in portal vein were increased rapidly in the development of AHNP. They were lower in hepatic vein (280.59 ± 20.02) and femoral artery (310.82 ± 7.97) than in portal vein (354.91 ± 25.50) (P < 0.05), and higher in femoral artery than in hepatic vein 6 h after the operation (P < 0.05). TNFα level in plasma was increased significantly when ET level in portal vein showed no increase.

CONCLUSION: Pancreas, spleen, liver, intestinal tract and lung are the main organs to produce TNFα, and liver is also an important site for TNFα uptake in the development of AHNP. Plasma endotoxin is not a trigger for TNFα release in rats with AHNP.

Keywords: tumor necrosis factor/blood, endotoxius/blood, pancreatitis/blood, acute diseases, disease models, animal, enzyme-linked immunosorbent assay, pancreas/pathology, necrosis