Published online Apr 15, 1998. doi: 10.3748/wjg.v4.i2.100
Revised: August 21, 1997
Accepted: September 10, 1997
Published online: April 15, 1998
AIM: To partially isolate and identify hepatic stimulator substance mRNA from human fetal liver tissues.
METHODS: The poly (A) mRNA was extracted from human fetal liver tissues of 4-5 month gestation, fractionated by size on sucrose gradient centrifugation, translated into protein from each fraction in vitro and then its products were tested for HSS activity.
RESULTS: Twenty-two 500 μg total RNA was obtained from human fetal liver tissues and pooled. mRNA of 420 μg was yielded, processed by oligo (dT)-cellulose column chromatography, then was size-fractionated by ultracentrifution on a continuous sucrose density gradient (5%-25%), and separated into 18 fractions. Translated products of mRNA in fraction 8 and 9 could produce a two-fold increase in the incorporation of 3H-TdR into DNA of SMMC-7721 hepatoma cells and in a heat resistant and organ-specific way.
CONCLUSION: The partially purified HSS mRNA was obtained and this would facilitate the cloning of HSS using expression vectors.