Original Research
Copyright ©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 15, 1997; 3(1): 41-42
Published online Mar 15, 1997. doi: 10.3748/wjg.v3.i1.41
Analysis of lactate dehydrogenase activities and isoenzyme patterns in colorectal cancer tissues
Chun-Hua Zhao, Chun-Ying Jiang, Yu-Yi Zhang, Xian-Xi Liu, Dao-Chun Luo, Xiao-Ting Zhang, Yu-Qin Lin
Chun-Hua Zhao, Yu-Yi Zhang, Xian-Xi Liu, Dao-Chun Luo, Yu-Qin Lin, The Department of Biochemistry, Shandong Medical University, Jinan 250012, Shandong Province, China
Chun-Ying Jiang, Xiao-Ting Zhang, Shandong Traditional Hospital, Jinan 250012, Shandong Province, China
Chun-Hua Zhao, with 8 published papers, Lecturer of Biochemistry
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Chun-Hua Zhao, The Department of Biochemistry, Shandong Medical University, Jinan 250012, Shandong Province, China
Telephone: +86-531-2952424-693
Received: July 1, 1996
Revised: September 1, 1996
Accepted: January 1, 1997
Published online: March 15, 1997
Abstract

AIM: To investigate the relationship between lactate dehydrogenase (LDH) activity or LDH isoenzyme patterns and the pathogenesis of colorectal cancer.

METHODS: Activities of tissue LDH and LDH isoenzyme patterns in 16 patients with colorectal cancer were assayed using spectrophotometric procedures and agarose gel electrophoresis, respectively.

RESULTS: The total and specific activities of LDH were significantly higher in colorectal cancer tissues than those in adjacent noncancerous tissues (P < 0.001). The LDH isoenzyme pattern was also different from that in the control. The percentage of LDH5 doubled and the ratio of LDH4 + LDH5/LDH1 + LDH2 was 3.6 ± 1.4 in cancer tissue, significantly greater than in the control.

CONCLUSIONS: The increased LDH activity in colorectal cancer tissues resulted mainly from the increased LDH5, suggesting that the alteration of LDH activity and isoenzyme patterns were related to the pathogenesis of colorectal cancer.

Keywords: Colonic neoplasms; Rectal neoplasms; Lactate dehydrogenase; Lactate dehydrogenase isoenzymes