Published online Mar 7, 2020. doi: 10.3748/wjg.v26.i9.933
Peer-review started: December 5, 2019
First decision: December 30, 2019
Revised: January 8, 2020
Accepted: January 19, 2020
Article in press: January 19, 2020
Published online: March 7, 2020
As the most common biliary malignancy, gallbladder cancer (GC) is an elderly-biased disease. Although extensive studies have elucidated the molecular mechanism of microRNA 182 (miR-182) and reversion-inducing-cysteine-rich protein with kazal motifs (RECK) in various cancers, the specific role of exosomal miR-182 and RECK in GC remains poorly understood.
To explore the relationship between exosomal miR-182/RECK and metastasis of GC.
Paired GC and adjacent normal tissues were collected from 78 patients. Quantitative polymerase chain reaction was employed to detect miR-182 and exosomal miR-182 expression, and Western blotting was conducted to determine RECK expression. In addition, the effects of exosomal miR-182/RECK on the biological function of human GC cells were observed. Moreover, the double luciferase reporter gene assay was applied to validate the targeting relationship between miR-182 and RECK.
Compared with normal gallbladder epithelial cells, miR-182 was highly expressed in GC cells, while RECK had low expression. Exosomal miR-182 could be absorbed and transferred by cells. Exosomal miR-182 inhibited RECK expression and promoted the migration and invasion of GC cells.
Exosomal miR-182 can significantly promote the migration and invasion of GC cells by inhibiting RECK; thus miR-182 can be used as a therapeutic target for GC.
Core tip: Gallbladder cancer (GC) is the most common biliary malignancy that mostly affects the elderly. At present, however, the specific role of exosomal miR-182 and RECK in GC remains unknown. The results of this study indicated that exosomal miR-182 could markedly promote the migration and invasion of GC cells by inhibiting RECK. Therefore, miR-182 could serve as a therapeutic target for GC.