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©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
Correlation between colonic secretion and colonic motility in rats: Role of ghrelin
Hsien-Hao Huang, Ching-Heng Ting, Yu-Fong Syu, Shi-Chuan Chang, Chih-Yen Chen
Hsien-Hao Huang, Institute of Clinical Medicine, National Yang-Ming University School of Medicine, Taipei 112, Taiwan
Hsien-Hao Huang, Department of Emergency Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan
Ching-Heng Ting, Department of Pathology, Mackay Memorial Hospital, Taipei 104, Taiwan
Yu-Fong Syu, Department of Family Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan
Shi-Chuan Chang, Institute of Emergency and Critical Medicine, National Yang-Ming University School of Medicine, Taipei 112, Taiwan
Shi-Chuan Chang, Department of Chest Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan
Chih-Yen Chen, Division of Gastroenterology and Hepatology, Department of Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan
Chih-Yen Chen, Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei 112, Taiwan
Chih-Yen Chen, Taiwan Association for the Study of Small Intestinal Diseases, Guishan 333, Taiwan
Author contributions: Huang HH and Chen CY contributed to the conception and design of the study; Huang HH, Ting CH, Syu YF, and Chen CY contributed to the acquisition, analysis and interpretation of data; all authors drafted the article, made critical revisions related to the intellectual content of the manuscript, and approved the final version of the article to be published.
Supported by the Taiwan Ministry of Science and Technology No. NSC 95-2314-B-075-013-MY2 and No. NSC 95-2314-B-010-098-MY2 to Chih-Yen Chen.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC), Taipei Veterans General Hospital.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: The statistical code and dataset are available from the corresponding author at chency@vghtpe.gov.tw.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Chih-Yen Chen, MD, PhD, Division of Gastroenterology and Hepatology, Taipei Veterans General Hospital, 201, Sec. 2, Shih-Pai Road, Taipei 112, Taiwan. chency@vghtpe.gov.tw
Telephone: +886-2-28712121-3763 Fax: +886-2-28711058
Received: June 28, 2016
Peer-review started: July 2, 2016
First decision: October 11, 2016
Revised: October 24, 2016
Accepted: November 14, 2016
Article in press: November 16, 2016
Published online: December 14, 2016
AIM
To explore the relationship between colonic secretory function and colonic motility.
METHODS
Using a rat model chronically implanted with intracerebroventricular (ICV) and cecal catheters, we validated the correlation between colonic secretion and colonic motor functions, as well as the role of ICV injection volume.
RESULTS
Compared to saline controls (5 μL/rat), ICV acyl ghrelin at 1 nmol/5 μL enhanced the total fecal weight, accelerated the colonic transit time, and increased the fecal pellet output during the first hour post-injection, while ICV des-acyl ghrelin at 1 nmol/5 μL only accelerated the colonic transit time. These stimulatory effects on colonic motility and/or secretion from acyl ghrelin and des-acyl ghrelin disappeared when the ICV injection volume increased to 10 μL compared with saline controls (10 μL/rat). Additionally, the ICV injection of 10 μL of saline significantly shortened the colonic transit time compared with the ICV injection of 5 μL of saline. The total fecal weight during the first hour post-injection correlated with the colonic transit time and fecal pellet output after the ICV injection of acyl ghrelin (1 nmol/5 μL), whereas the total fecal weight during the first hour post-injection correlated with the fecal pellet output but not the colonic transit time after the ICV injection of des-acyl ghrelin (1 nmol/5 μL).
CONCLUSION
Colonic secretion does not always correlate with colonic motility in response to different colonic stimulations. Acyl ghrelin stimulates colonic secretion.
Core tip: The colokinetic effects of acyl ghrelin and des-acyl ghrelin depend on the intracerebroventricular (ICV) injection volume, and the acute increase of the ICV volume accelerates the colonic transit time. In addition, acyl ghrelin, rather than des-acyl ghrelin, stimulates colonic secretion. Colonic secretion does not always correlate with colonic motility in response to different colonic stimulations.
