Basic Study
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 21, 2015; 21(11): 3245-3255
Published online Mar 21, 2015. doi: 10.3748/wjg.v21.i11.3245
MicroRNA-1290 promotes esophageal squamous cell carcinoma cell proliferation and metastasis
Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi
Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Ming Li, Department of Gastroenterology, Friendliness Hospital of Yangzhou, Yangzhou 225009, Jiangsu Province, China
Zhi-Mei Zhang, Department of Gastroenterology, First People’s Hospital of Lianyungang, Lianyungang 222002, Jiangsu Province, China
Author contributions: Li M designed the research; He XY, Zhang ZM, Li S and Ren LH performed the research; Cao RS and Feng YD collected the clinical data; Ji YL, Shi RH and Zhao Y analyzed the data; and Li M wrote the paper.
Supported by grants from Innovative Team Project of Jiangsu Province, China, No. CXZZ11_0705.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Rui-Hua Shi, MD, PhD, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guang Zhou Road, Gulou District, Nanjing 210029, Jiangsu Province. China. ruihuashi@126.com
Telephone: +86-25-83674636 Fax: +86-25-83718836
Received: August 8, 2014
Peer-review started: August 9, 2014
First decision: September 27, 2014
Revised: October 11, 2014
Accepted: December 16, 2014
Article in press: December 16, 2014
Published online: March 21, 2015
Abstract

AIM: To investigate the biological role of miR-1290 in esophageal squamous cell carcinoma (ESCC) progression and invasion and the underlying mechanism.

METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate miR-1290 expression in ESCC tissue samples. The roles of miR-1290 in cell proliferation, migration and invasion were identified using miR-1290 mimic-transfected cells. In addition, the regulatory effect of miR-1290 on suppressor of cancer cell invasion (SCAI) was evaluated using qRT-PCR, Western blot analysis and a dual luciferase reporter assay.

RESULTS: miR-1290 was significantly upregulated in ESCC tissue samples compared with normal adjacent tissues (9.213 ± 1.150 vs 1.000 ± 0.0), (P < 0.01). Upregulation of miR-1290 was associated with tumor differentiation (P = 0.021), N classification (P = 0.006) and tumor-node-metastasis stage (P = 0.021) in ESCC patients. Moreover, ectopic miR-1290 expression potently promoted ESCC cell growth (P < 0.01), migration (P < 0.01) and invasion (P < 0.01) in vitro. miR-1290 overexpression in ESCC cell lines decreased SCAI expression at the translational level and reduced SCAI-driven luciferase-reporter activity (P < 0.01).

CONCLUSION: Our findings suggested that miR-1290 may play an oncogenic role in cellular processes of ESCC.

Keywords: MicroRNA, MiR-1290, Esophageal squamous cell carcinoma, Suppressor of cancer cell invasion, Invasion, Metastasis

Core tip: In this study, we reported the clinical significance and biological effects of miR-1290 in esophageal squamous cell carcinoma (ESCC). We found that miR-1290 was significantly up-regulated in ESCC tissues. Moreover, we showed that ectopic expression of miR-1290 significantly promoted ESCC cell growth, migration and invasion. Further investigation revealed that suppressor of cancer cell invasion was a downstream target of miR-1290.