Experimental Papers
Copyright ©The Author(s) 1996. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 1996; 2(4): 187-189
Published online Dec 15, 1996. doi: 10.3748/wjg.v2.i4.187
Detecting the localization of hepatitis B and C viruses in hepatocellular carcinoma by double in situ hybridization
Yan-Jun Liu, Wen-Ming Cong, Tian-Pei Xie, Hao Wang, Feng Shen, Ya-Jun Guo, Han Chen, Meng-Chao Wu
Yan-Jun Liu, Wen-Ming Cong, Tian-Pei Xie, Hao Wang, Feng Shen, Ya-Jun Guo, Han Chen, Meng-Chao Wu, Eastern Institute of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China
Author contributions: All authors contributed equally to the work.
Supported by The National Natural Science Foundation of China, No. 39070386.
Correspondence to: Yan-Jun Liu, MD, PhD, Assistant Professor, Eastern Institute of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China
Telephone: +86-21-65566349
Received: August 10, 1996
Revised: September 25, 1996
Accepted: November 10, 1996
Published online: December 15, 1996
Abstract

AIM: To detect whether hepatitis B virus (HBV) and hepatitis C virus (HCV) can infect the same host cell.

METHODS: We established a method of non-isotope double in situ hybridization by combining a degoxigenin-labeled probe with a biotin-labeled probe, and detected HBV and HCV infections in 10 cases of hepatocellular carcinoma.

RESULTS: It was found that HBV and HCV can infect the same host cell, which is a unique biological phenomenon.

CONCLUSION: Infections with HBV and HCV might not always follow the rule of “viral interference”, which provides a new clue for investigating the synergistic action of HBV and HCV in the pathogenesis of hepatocellular carcinoma.

Keywords: In situ hybridization, Liver neoplasms, Hepatitis B virus, Hepatitis C virus