Copyright
©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
Special AT-rich sequence-binding protein 1 promotes cell growth and metastasis in colorectal cancer
Xue-Feng Fang, Zhi-Bo Hou, Xin-Zheng Dai, Cong Chen, Jing Ge, Hong Shen, Xiao-Feng Li, Li-Ke Yu, Ying Yuan
Xue-Feng Fang, Hong Shen, Ying Yuan, Xiao-Fang Li, Department of Medical Oncology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310000, Zhejiang Province, China
Zhi-Bo Hou, Li-Ke Yu, First Department of Respiratory Medicine, Nanjing Chest Hospital, Nanjing 210029, Jiangsu Province, China
Xin-Zheng Dai, Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing 210029, Jiangsu Province, China
Cong Chen, Department of Gynecology of Traditional Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Jing Ge, Department of Endocrinology, Jiangsu Provincial Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Author contributions: Fang XF, Hou ZB and Dai XZ contributed equally to this work; Hou ZB, Dai XZ and Chen C carried out the molecular genetic studies; Chen C, Ge J and Li XF participated in the animal study; Hou ZB, Chen C and Ge J analyzed final data; Fang XF, Hou ZB and Shen H drafted the manuscript; Yuan Y designed this research; all authors read and approved the final manuscript.
Supported by The National Natural Science Foundation of China, No. 81101580
Correspondence to: Dr. Ying Yuan, Department of Medical Oncology, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310000, Zhejiang Province, China. yuanying1999@zju.edu.cn
Telephone: +86-571-87784795 Fax: +86-571-87767088
Received: December 2, 2012
Revised: January 13, 2013
Accepted: February 2, 2013
Published online: April 21, 2013
AIM: To evaluate the expression of special AT-rich sequence-binding protein 1 (SATB1) gene in colorectal cancer and its role in colorectal cancer cell proliferation and invasion.
METHODS: Immunohistochemistry was used to detect the protein expression of SATB1 in 30 colorectal cancer (CRC) tissue samples and pair-matched adjacent non-tumor samples. Cell growth was investigated after enhancing expression of SATB1. Wound-healing assay and Transwell assay were used to investigate the impact of SATB1 on migratory and invasive abilities of SW480 cells in vitro. Nude mice that received subcutaneous implantation or lateral tail vein were used to study the effects of SATB1 on tumor growth or metastasis in vivo.
RESULTS: SATB1 was over-expressed in CRC tissues and CRC cell lines. SATB1 promotes cell proliferation and cell cycle progression in CRC SW480 cells. SATB1 overexpression could promote cell growth in vivo. In addition, SATB1 could significantly raise the ability of cell migration and invasion in vitro and promote the ability of tumor metastasis in vivo. SATB1 could up-regulate matrix metalloproteases 2, 9, cyclin D1 and vimentin, meanwhile SATB1 could down-regulate E-cadherin in CRC.
CONCLUSION: SATB1 acts as a potential growth and metastasis promoter in CRC. SATB1 may be useful as a therapeutic target for CRC.
