Brief Article
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World J Gastroenterol. Dec 21, 2011; 17(47): 5172-5176
Published online Dec 21, 2011. doi: 10.3748/wjg.v17.i47.5172
Five methods for detection of Helicobacter pylori in the Turkish population
Orhan Cem Aktepe, İhsan Hakkı Çiftçi, Birol Şafak, İhsan Uslan, Fatma Hüsniye Dilek
Orhan Cem Aktepe, İhsan Hakkı Çiftçi, Department of Microbiology, Faculty of Medicine, Afyon Kocatepe University, Ali Çetinkaya Campus, 03200 Afyonkarahisar, Turkey
Birol Şafak, Microbiology Laboratory, Atatürk State Hospital, Gaziosmanpaşa Mah., Soma Cad., 10100 Balıkesir, Turkey
İhsan Uslan, Gastroenterology Department, Kütahya State Hospital, Afyon Cad., 43100 Kütahya, Turkey
Fatma Hüsniye Dilek, Department of Pathology, Faculty of Medicine, Afyon Kocatepe University, Ali Çetinkaya Campus, 03200 Afyonkarahisar, Turkey
Author contributions: Aktepe OC, Çiftçi İH and Dilek FH designed the research; Aktepe OC, Çiftçi İH, Şafak B and Uslan İ performed the research; Aktepe OC contributed to new analytic tools; Çiftçi İH and Dilek FH analyzed the data; and Aktepe OC and Çiftçi İH wrote the paper.
Correspondence to: Orhan Cem Aktepe, Associated Professor, Department of Microbiology, Faculty of Medicine, Afyon Kocatepe University, Ali Çetinkaya Campus, 03200 Afyonkarahisar, Turkey. aktepef@hotmail.com
Telephone: +90-272-2463304 Fax: +90-272-2463300
Received: January 23, 2011
Revised: April 19, 2011
Accepted: April 26, 2011
Published online: December 21, 2011
Abstract

AIM: To compare culture analysis, Helicobacter pylori (H. pylori) stool antigen (HpSA) test, polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) for H. pylori detection.

METHODS: One hundred and thirty-two consecutive adult dyspeptic patients receiving diagnostic endoscopy at the department of gastroenterology were enrolled in this study. Culture and histological examination were performed on biopsy specimens. PCR and FISH tests were applied to histopathological samples. Stool samples that were simultaneously collected were tested for the H. pylori antigen using the HpSA test and bacterial DNA using stool PCR.

RESULTS: H. pylori was positively identified by histological examination in 85/132 (64.4%) of the patients, while positive samples were found in 56 (42.4%), 64 (48.5%), 98 (74.2%), 28 (21.2%) and 81 (61.4%) of the patients by culture, HpSA, PCR, stool PCR and FISH methods, respectively. The results of the culture, biopsy PCR, HpSA and FISH tests, with the exception of the stool PCR, were found to correlate with the histological examination as a gold standard.

CONCLUSION: The HpSA test is a rapid, simple, and noninvasive test for monitoring therapy. FISH is an accurate, rapid, cost-effective, and easy-to-use test for H. pylori detection.

Keywords: Helicobacter pylori; Histology; Polymerase chain reaction; Helicobacter pylori stool antigen; Fluorescence in situ hybridization