Son KR, Chung SY, Kim HC, Kim HS, Choi SH, Lee JM, Moon WK. MRI of magnetically labeled mesenchymal stem cells in hepatic failure model. World J Gastroenterol 2010; 16(44): 5611-5615 [PMID: 21105195 DOI: 10.3748/wjg.v16.i44.5611]
Corresponding Author of This Article
Woo Kyung Moon, MD, Department of Radiology, Seoul National University College of Medicine, Institute of Radiation Medicine, Seoul National University Medical Research Center, and Clinical Research Institute, Seoul National University Hospital, Seoul, #28 Yongon-dong, Chongno-gu, Seoul 110-744, South Korea. moonwk@radcom.snu.ac.kr
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World J Gastroenterol. Nov 28, 2010; 16(44): 5611-5615 Published online Nov 28, 2010. doi: 10.3748/wjg.v16.i44.5611
MRI of magnetically labeled mesenchymal stem cells in hepatic failure model
Kyu Ri Son, Se Young Chung, Hyo-Cheol Kim, Hoe Suk Kim, Seung Hong Choi, Jeong Min Lee, Woo Kyung Moon
Kyu Ri Son, Department of Radiology, Korea University Medical Center, Anam Hospital, Seoul 136-705, South Korea
Se Young Chung, Hyo-Cheol Kim, Hoe Suk Kim, Seung Hong Choi, Jeong Min Lee, Woo Kyung Moon, Department of Radiology, Seoul National University College of Medicine, Institute of Radiation Medicine, Seoul National University Medical Research Center, and Clinical Research Institute, Seoul National University Hospital, Chongno-gu, Seoul 110-744, South Korea
Author contributions: Moon WK designed the study; all authors performed the experimental study; Son KR, Chung SY, Kim HC, Kim HS, Choi SH and Lee JM analyzed the data; Son KR, Kim HC and Moon WK wrote the paper; all authors reviewed the paper.
Supported by Innovative Research Institute for Cell Therapy, No. A062260; Contract grant sponsor: National R&D Program for Cancer Control, Ministry of Health and Welfare, No. 0920030; Basic Science Research Program Through the National Research Foundation funded by the Ministry of Education, Science and Technology, No. 20090080219; Korea Healthcare Technology R&D Projects, Ministry for Health, Welfare and Family Affairs, Republic of Korea, No. A091001
Correspondence to: Woo Kyung Moon, MD, Department of Radiology, Seoul National University College of Medicine, Institute of Radiation Medicine, Seoul National University Medical Research Center, and Clinical Research Institute, Seoul National University Hospital, Seoul, #28 Yongon-dong, Chongno-gu, Seoul 110-744, South Korea. moonwk@radcom.snu.ac.kr
Telephone: +82-2-20722584 Fax: +82-2-7436385
Received: April 26, 2010 Revised: June 9, 2010 Accepted: June 15, 2010 Published online: November 28, 2010
Abstract
AIM: To track intravascularly transplanted mesenchymal stem cells (MSCs) labeled with superparamagnetic iron oxide (SPIO) by using magnetic resonance imaging (MRI) in an experimental rabbit model of hepatic failure.
METHODS: Human MSCs labeled with FDA-approved SPIO particles (Feridex) were transplanted via the mesenteric vein into rabbits (n = 16) with carbon tetrachloride-induced hepatic failure. Magnetic resonance (MR) examinations were performed with a 3.0 T clinical scanner immediately before and 2 h and 1, 3, and 7 d after transplantation. Signal intensity (SI) changes on T2*-weighted MRI were measured, and correlation between MR findings and histomorphologic findings was also investigated.
RESULTS: SI on T2*-weighted MRI decreased significantly in the liver 2 h after injection of human MSCs and returned gradually to the levels found before injection in 7 d. Changes in SI in the liver at 2 h, 1, 3, and 7 d were 41.87% ± 9.63%, 10.42% ± 4.3%, 5.12% ± 1.9%, 3.75% ± 1.2%, respectively (P < 0.001). Histologic analyses confirmed the presence of MSCs in the liver, localized mainly in the sinusoids in early period (2 h and 1 d) and concentrated to the border zone in late period (3 and 7 d). The number of iron-positive cells in the liver at 2 h and on 1, 3 and 7 d after transplantation was 29.2 ± 4.8, 10.1 ± 3.7, 6.7 ± 2.2, and 5.8 ± 2.1, respectively (P = 0.013).
CONCLUSION: Intravascularly injected SPIO-labeled MSCs in an experimental rabbit model of hepatic failure can be detected and followed with MRI.
