Original Articles
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Mar 21, 2009; 15(11): 1346-1352
Published online Mar 21, 2009. doi: 10.3748/wjg.15.1346
Curcumin suppresses PPARδ expression and related genes in HT-29 cells
Jin-Bo Wang, Li-Li Qi, Shui-Di Zheng, Heng-Zheng Wang, Tian-Xing Wu
Jin-Bo Wang, Tian-Xing Wu, Department of Chemistry, College of Science, Zhejiang University, Hanzhou 310027, Zhejiang Province, China
Jin-Bo Wang, Li-Li Qi, Shui-Di Zheng, Heng-Zheng Wang, Tian-Xing Wu, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, Zhejiang Province, China
Author contributions: Wang JB, Wu TX designed the study; Wang JB, Qi LL, Zheng SD and Wang HZ performed the research and analyzed the data; Wang JB wrote the paper.
Correspondence to: Tian-Xing Wu, Ningbo Institute of Technology, Zhejiang University, NO. 1 Xuefu Road, Ningbo 315100, Zhejiang Province, China. wangjbmail@yahoo.com.cn
Telephone: +86-574-88229516
Fax: +86-574-88229516
Received: November 17, 2008
Revised: January 12, 2009
Accepted: January 19, 2009
Published online: March 21, 2009
Abstract

AIM: To investigate the effects of curcumin on the expression of peroxisome proliferator-activated receptorδ (PPARδ) and related genes in HT-29 cells.

METHODS: HT-29 cells were treated with curcumin (0-80 &mgr;mol/L) for 24 h. The effects of curcumin on the morphology of HT-29 cells were studied by Hoechst 33342 staining. The activity of caspase-3 was determined using DEVD-pNA as substrate. The levels of peroxisome PPARδ, 14-3-3epsilon and vascular endothelial growth factor (VEGF) in HT-29 cells were determined by Western blotting analysis and their mRNA expression was determined by real-time quantitative RT-PCR.

RESULTS: Treatment with 10-80 &mgr;mol/L curcumin induced typical features of apoptosis and activated the caspase-3 in HT-29 cells. The expression of PPARδ, 14-3-3epsilon and VEGF was reduced and the activity of β-catenin/Tcf-4 signaling was inhibited by curcumin treatment.

CONCLUSION: Curcumin can induce apoptosis of HT-29 cells and down-regulate the expression of PPARδ, 14-3-3epsilon and VEGF in HT-29.

Keywords: Curcumin, 14-3-3epsilon, Peroxisome proliferator-activated receptorδ, Vascular endothelial growth factor, HT-29 cells