Zhang XP, Zhang J, Ren Z, Feng GH, Zhu W, Cai Y, Yang QJ, Ju TF, Xie Q, Yuan WQ. Study on protecting effects of Baicalin and Octreotide on hepatic injury in rats with severe acute pancreatitis. World J Gastroenterol 2008; 14(42): 6551-6559 [PMID: 19030211 DOI: 10.3748/wjg.14.6551]
Corresponding Author of This Article
Guang-Hua Feng, Director, Department of General Surgery, Hangzhou First People’s Hospital, 261 Huansha Road, Hangzhou 310006, Zhejiang Province, China. zxp99688@vip.163.com
Article-Type of This Article
Rapid Communication
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World J Gastroenterol. Nov 14, 2008; 14(42): 6551-6559 Published online Nov 14, 2008. doi: 10.3748/wjg.14.6551
Study on protecting effects of Baicalin and Octreotide on hepatic injury in rats with severe acute pancreatitis
Xi-Ping Zhang, Jie Zhang, Zheng Ren, Guang-Hua Feng, Wei Zhu, Yang Cai, Qi-Jun Yang, Tong-Fa Ju, Qi Xie, Wen-Qin Yuan
Xi-Ping Zhang, Guang-Hua Feng, Wei Zhu, Yang Cai, Qi-Jun Yang, Tong-Fa Ju, Qi Xie, Wen-Qin Yuan, Department of General Surgery, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China
Jie Zhang, First College of Clinical Medicine, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, Zhejiang Province, China
Zheng Ren, Department of General Surgery, Second Affiliated Hospital Medical College, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Author contributions: Zhang XP designed the research; Feng GH contributed to manage and guide the research; Zhang XP, Zhang J, Ren Z, Zhu W, Cai Y, Yang QJ, Ju TF, Xie Q, Yuan WQ performed the research; Zhang XP, and Zhang J analyzed the data; Zhang XP, Zhang J and Ren Z wrote the paper.
Supported by Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang province, No. 2003C130 and No. 2004C142; Foundation Project For Medical Science and Technology of Zhejiang province, No. 2003B134; Grave Foundation Project for Technological and Development of Hangzhou, No. 2003123B19; Intensive Foundation Project for Technology of Hangzhou, No. 2004Z006; Foundation Project for Medical Science and Technology of Hangzhou, No. 2003A004; and Foundation Project for Technology of Hangzhou, No. 2005224
Correspondence to: Guang-Hua Feng, Director, Department of General Surgery, Hangzhou First People’s Hospital, 261 Huansha Road, Hangzhou 310006, Zhejiang Province, China. zxp99688@vip.163.com
Telephone: +86-571-87065701 Fax: +86-571-87914773
Received: June 9, 2008 Revised: August 11, 2008 Accepted: August 18, 2008 Published online: November 14, 2008
Abstract
AIM: To investigate the protective effects and mechanisms of Baicalin and Octreotide on hepatic injury in rats with severe acute pancreatitis (SAP).
METHODS: The SAP rat models were prepared and randomly assigned to the model control group, Baicalin treated group, and Octreotide treated group while other healthy rats were assigned to the sham-operated group. Rat mortality, levels of ALT, AST, liver and pancreas pathological changes in all groups were observed at 3, 6 and 12 h after operation. Tissue microarray (TMA) sections of hepatic tissue were prepared to observe expression levels of Bax, Bcl-2 protein and Caspase-3, and changes of apoptotic indexes.
RESULTS: Rat survival at 12 h, expression levels of Bax, Caspase-3 protein and apoptotic indexes of liver were all significantly higher in treated groups than in model control group. While the liver and pancreas pathological scores, contents of ALT, AST, and expression levels of Bcl-2 protein were all lower in treated groups than in the model control group.
CONCLUSION: Both Baicalin and Octreotide can protect rats with SAP by decreasing the contents of ALT, AST and expression levels of Bcl-2 protein, and improving the expression levels of Bax protein, Caspase-3 protein, and inducing apoptosis.
