Colorectal Cancer
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Oct 14, 2008; 14(38): 5827-5833
Published online Oct 14, 2008. doi: 10.3748/wjg.14.5827
Antitumor bioactivity of adenovirus-mediated p27mt in colorectal cancer cell line SW480
Ze-Qun Sun, Chang-Sheng Deng, Shao-Yong Xu, Yong Du
Ze-Qun Sun, Chang-Sheng Deng, Gastroenterology Department of Zhongnan Hospital, Wuhan University, East Lake Road, Wuhan 430000, Hubei Province, China
Shao-Yong Xu, Yong Du, Gastroenterology Department of Renmin Hospital, Yunyang Medical College, Shiyan 442000, Hubei Province, China
Author contributions: Sun ZQ, Deng CS, Xu SY and Du Y designed the research; Sun ZQ and Du Y performed the research; Sun ZQ, Deng CS, Xu SY and Du Y analyzed data; and Sun ZQ and Deng CS wrote the paper.
Supported by Grant from the Natural Science Foundation of Hubei Province, NO.2003ABA193
Correspondence to: Chang-Sheng Deng, Gastroenterology Department of Zhongnan Hospital, Wuhan University, East Lake Road, Wuhan 430000, Hubei Province, China. wwwszq@163.com
Telephone: +86-27-87336067 Fax: +86-27-67813079
Received: April 9, 2008
Revised: August 12, 2008
Accepted: August 19, 2008
Published online: October 14, 2008
Abstract

AIM: To explore the antitumor bioactivity of adenovirus-mediated mutant type p27kip1 gene in a colorectal cancer cell line SW480.

METHODS: We constructed recombinant adenovirus vector expressing a mutant type p27kip1 gene (ad-p27mt), with mutation of Thr-187/Pro-188 (ACGCCC) to Met-187/Ile-188 (ATGATC), and transduced into SW480 cells. Then we detected expression of p27, Bcl-2 and Bax protein in the transductants by Western blotting, cell cycle of transductants by a digital flow cytometric system, migrating potential with Boyden Chamber and SW480 tumor cell growth inhibition in vitro and in vivo.

RESULTS: We found that a recombinant adenovirus vector of expressing ad-p27mt, with mutation of Thr-187/Pro-188 (ACGCCC) to Met-187/Ile-188 (ATGATC) has potent inhibition of SW480 tumor cell growth in vitro and in vivo. Furthermore, ad-p27mt induced cell apoptosis via regulating bax and bcl-2 expressions, and G1/S arrest in SW480 cells and inhibited cell migration.

CONCLUSION: ad-p27mt has a strong anti-tumor bioactivity and has the potential to develop into new therapeutic agents for colorectal cancer.

Keywords: p27, Adenovirus, Antitumor bioactivity, Colorectal cancer