Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 14, 2005; 11(6): 912-916
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.912
Alcohol dehydrogenase: A potential new marker for diagnosis of intestinal ischemia using rat as a model
Upendra R Gumaste, Mukund M Joshi, Devendra T Mourya, Pradip V Barde, Ghanshyam K Shrivastav, Vikram S Ghole
Upendra R Gumaste, Ghanshyam K Shrivastav, Vikram S Ghole, Division of Biochemistry, Department of Chemistry, University of Pune, Pune 411007, India
Devendra T Mourya, Pradip V Barde, Microbial Containment Complex, National Institute of Virology, Sus-Road, Pashan, Pune, Pune 411008, India
Mukund M Joshi, Department of Surgery, B J Medical College, Pune 411001 and Joshi Clinic and Health Sciences Foundation, Pune 411005, India
Author contributions: All authors contributed equally to the work.
Supported by Department of Science and Technology, Ministry of Science and Technology, Government of India, India
Correspondence to: Vikram Ghole, Division of Biochemistry,Department of Chemistry, University of Pune, Pune 411007, India.
Telephone: +91-20-25601225 Fax: +91-20-25691728
Received: September 29, 2004
Revised: October 1, 2004
Accepted: October 18, 2004
Published online: February 14, 2005

AIM: Intestinal ischemia (Ii) is an abdominal emergency due to blockade of the superior mesenteric artery resulting in 60-100% mortality if diagnosed late. Changes in several biochemical parameters such as D (-)-lactate, Creatinine kinase isoenzymes and lactate dehydrogenase suggested for early diagnosis, lack specificity and sensitivity. Therefore a biochemical parameter with greater sensitivity needs to be identified.

METHODS: Wistar male rats were randomly assigned into two groups; control sham operated (n = 24) and ischemic test (n = 24) group. Superior mesenteric arterial occlusion was performed in the ischemic test group for 1 h. Alcohol dehydrogenase (ADH) was estimated in blood from portal vein, right ventricle of heart, dorsal aorta (DA) and inferior vena cava (IVC). The Serum glutamic acid pyruvate transaminase (SGPT) was also estimated in blood from portal vein and right ventricle of heart.

RESULTS: A significant increase (P<0.001) in the levels of ADH in both portal blood as well as heart blood of the test group (232.72±99.45 EU and 250.85±95.14 EU, respectively) as compared to the control group (46.39±21.69 EU and 65.38±30.55 EU, respectively) were observed. Similarly, increased levels of ADH were observed in blood samples withdrawn from DA and IVC in test animals (319.52±80.14 EU and 363.90±120.68 EU, respectively) as compared to the control group (67.68±63.22 EU and 72.50±58.45 EU, respectively). However, in test animals there was significant increase in SGPT in portal blood (P = 0.054) without much increase in heart blood.

CONCLUSION: Significant increase in the levels of ADH in portal and heart blood within 1 h of SMA occlusion without increase in SGPT in heart blood, suggests that the origin of ADH is from ischemic intestine and not from liver. Similarly, raised ADH levels were found in DA and IVC as well. IVC blood does represent peripheral blood sample. A raised level of ADH in test animals confirms it to be a potential marker in the early diagnosis of Ii.

Keywords: Intestinal ischemia, ADH, Biochemical parameter