Effects of garlicin on apoptosis in rat model of colitis

doi:10.3748/wjg.v11.i29.4579

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 7, 2005; 11(29): 4579-4582
Published online Aug 7, 2005. doi: 10.3748/wjg.v11.i29.4579

Effects of garlicin on apoptosis in rat model of colitis

Xi-Ming Xu, Jie-Ping Yu, Xiao-Fei He, Jun-Hua Li, Liang-Liang Yu, Hong-Gang Yu

Xi-Ming Xu, Jie-Ping Yu, Jun-Hua Li, Liang-Liang Yu, Hong-Gang Yu, Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China

Xiao-Fei He, Department of Gastroenterology, the Affiliated Hospital of Xianning Medical College, Xianning 437100, Hubei Province, China

Author contributions: All authors contributed equally to the work.

Supported by the Educational Foundation of Hubei Province, No. 2002A04006

Correspondence to: Hong-Gang Yu, Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China. doctorxu120@sina.com

Telephone: +86-27-62954290

Received: October 30, 2004
Revised: December 23, 2004
Accepted: December 26, 2004
Published online: August 7, 2005

Abstract

AIM: To investigate the effects of garlicin on apoptosis and expression of bcl-2 and bax in lymphocytes in rat model of ulcerative colitis (UC).

METHODS: Healthy adult Sprague-Dawley rats of both sexes, weighing 180±30 g, were employed in the present study. The rat model of UC was induced by 2,4,6-trinitr-obenzene sulfonic acid (TNBS) enema. The experimental animals were randomly divided into garlicin treatment group (including high and low concentration), model control group, and normal control group. Rats in garlicin treatment group and model control group received intracolic garlicin daily at doses of 10.0 and 30.0 mg/kg and equal amount of saline respectively 24 h after colitis model was induced by alcohol and TNBS co-enema. Rats in normal control group received neither alcohol nor only TNBS but only saline enema in this study. On the 28^th d of the experiment, rats were executed, the expression of bcl-2 and bax protein was determined immunohistochemically and the apoptotic cells were detected by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate fluorescence nick end labeling (TUNEL) method. At the same time, the rat colon mucosal damage index (CMDI) was calculated.

RESULTS: In garlicin treatment group, the positive expression of bcl-2 in lymphocytes decreased and the number of apoptotic cells was more than that in model control group, CMDI was lower than that in model control group. The positive expression of bax in lymphocytes had no significant difference.

CONCLUSION: Garlicin can protect colonic mucosa against damage in rat model of UC induced by TNBS enema.

Keywords: Garlicin, Ulcerative colitis, Apoptosis, Bcl-2