Liver Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 7, 2005; 11(25): 3860-3865
Published online Jul 7, 2005. doi: 10.3748/wjg.v11.i25.3860
Activation of transcription factors NF-kappaB and AP-1 and their relations with apoptosis-associated proteins in hepatocellular carcinoma
Lin-Lang Guo, Sha Xiao, Ying Guo
Lin-Lang Guo, Sha Xiao, Ying Guo, Department of Pathology, Zhujiang Hospital, Guangzhou 210282, Guangdong Province, China Lin-Lang Guo, University of California, Davis Cancer Center, CA 95817, the United States
Author contributions: All authors contributed equally to the work.
Correspondence to: Lin-Lang Guo, MD, University of California, Davis Cancer Center, Suite 2300, Research Building III, 4645 Second Avenue, Sacramento, CA 95817, United States.
Telephone: +916-734-1479 Fax: +916-734-2361
Received: August 18, 2004
Revised: November 23, 2004
Accepted: November 29, 2004
Published online: July 7, 2005

AIM: To study the distribution pattern of transcription factors NF-κB and AP-1 and their relations with the expression of apoptosis associated-proteins Fas/FasL and ICH-1L/S in human hepatocellular carcinoma (HCC).

METHODS: We performed in situ hybridization and immunohistochemical techniques for NF-κB, AP-1, Fas/FasL and ICH-1 in 40 cases of human HCC along with corresponding nontumoral tissues and 7 cases of normal liver tissues.

RESULTS: Twenty-two (55%) and 25 (62.5%) of 40 cases for NF-κB and AP-1 were presented for nuclear or both nuclear and cytoplastic staining respectively, while less cases were presented for only cytoplastic staining for NF-κB (18%) and AP-1 (10%) in adjacent nontumoral tissues and negative staining in normal liver tissues. There was no statistically significant difference of NF-κB or AP-1 activation between well differentiated tumors and poorly differentiated tumors (P > 0.05). NF-κB activity is positively corresponded to AP-1 activation. The expression of ICH-1L/S was associated with the activation of NF-κB and AP-1 (P < 0.05), but no significant relationship was found between Fas/FasL and NF-κB or AP-1(P > 0.05).

CONCLUSION: Activation of both NF-κB and AP-1 may be required for ICH-1L/S-induced apoptosis in HCC, but not for Fas/FasL-mediated apoptosis. NF-κB and AP-1 may play important roles in the pathogenesis of human HCC.

Keywords: Hepatocellular carcinoma (HCC), Transcription factors, Apoptosis, Protein