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©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 28, 2005; 11(12): 1837-1842
Published online Mar 28, 2005. doi: 10.3748/wjg.v11.i12.1837
Published online Mar 28, 2005. doi: 10.3748/wjg.v11.i12.1837
Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Xiao-Nan Cui, Li Li, Ji-Wei Liu, Department of Oncology, the 1st Affilitate Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
Jian-Wu Tang, Li Hou, Bo Song, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Jian-Wu Tang, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China. jianwutang@163.net
Telephone: +86-411-4720021
Received: February 3, 2004
Revised: February 4, 2004
Accepted: February 21, 2004
Published online: March 28, 2005
Revised: February 4, 2004
Accepted: February 21, 2004
Published online: March 28, 2005
Abstract
AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.
RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.
CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.