Gastric Cancer
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 7, 2005; 11(1): 69-72
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.69
Blocking effects of genistein on cell proliferation and possible mechanism in human gastric carcinoma
Hong-Bin Cui, Xiao-Lin Na, Dan-Feng Song, Ying Liu
Hong-Bin Cui, Xiao-Lin Na, Dan-Feng Song, Department of Food Nutrition and Hygiene, Public Health College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Ying Liu, Department of Biotechnology, Harbin Business University, Harbin 150076, Heilongjiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 39970637
Correspondence to: Professor Hong-Bin Cui, 199 Dongdazhi Street, Nangang District, Harbin 150001, Heilongjiang Province, China. cuihb_h@public.hr.hl.cn
Telephone: +86-451-53639451 Fax: +86-451-53648617
Received: August 23, 2003
Revised: August 28, 2003
Accepted: October 27, 2003
Published online: January 7, 2005
Abstract

AIM: To study the blocking effects of genistein on cell proliferation cycle in human gastric carcinoma cells (SGC-7901) and the possible mechanism.

METHODS: MTT assay was applied in the detection of the inhibitory effects of genistein on cell proliferation. Flow cytometry was used to analyze the cell cycle distribution. Immunocytochemical technique and Western blotting were performed to detect the protein expression of cyclin D1, cyclin B1 and p21waf1/cip1.

RESULTS: Genistein significantly inhibited the growth and proliferation of human gastric carcinoma cells (SGC-7901). Seven days after treatment with different concentrations of genistein (2.5, 5.0, 10.0, 20.0 μg /mL), the growth inhibitory rates were 11.2%, 28.8%, 55.3%, 84.7% respectively and cell cycles were arrested at the G(2)/ M phase. Genistein decreased cyclin D1 protein expression and enhanced cyclin B1 and p21waf/cip1 protein expression in a concentration-dependent manner.

CONCLUSION: The growth and proliferation of SGC-7901 cells can be inhibited by genistein via blocking the cell cycle, with reduced expression of cyclin D1 and enhanced expression of cyclin B1 and p21waf/cip1 protein in the concentration range of 0-20 μg /mL.

Keywords: Gastric carcinoma, Genistein, Cell proliferation, Cell cycle