Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 1, 2004; 10(9): 1240-1245
Published online May 1, 2004. doi: 10.3748/wjg.v10.i9.1240
GSTT1, GSTM1 and CYP2E1 genetic polymorphisms in gastric cancer and chronic gastritis in a Brazilian population
Jucimara Colombo, Andréa Regina Baptista Rossit, Alaor Caetano, Aldenis Albaneze Borim, Durval Wornrath, Ana Elizabete Silva
Jucimara Colombo, Ana Elizabete Silva, Departamento de Biologia, UNESP-Campus de São José do Rio Preto-SP, Brazil
Andréa Regina Baptista Rossit, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina, FAMERP, São José do Rio Preto, SP, Brazil
Alaor Caetano, Aldenis Albaneze Borim, Hospital de Base, Faculdade de Medicina, FAMERP, São José do Rio Preto, SP, Brazil
Durval Wornrath, Fundação Pio XII, Barretos, Brazil
Author contributions: All authors contributed equally to the work.
Supported by Brazilian Agency CAPES.
Correspondence to: Ana Elizabete Silva, Departamento de Biologia, UNESP - Campus de São José do Rio Preto, Rua Cristovão Colombo, 2265, 15054-000 - São José do Rio Preto, SP, Brazil. anabete@bio.ibilce.unesp.br
Telephone: +55-17-2212384 Fax: +55-17-2212390
Received: September 23, 2003
Revised: December 1, 2003
Accepted: December 30, 2003
Published online: May 1, 2004
Abstract

AIM: To test the hypothesis that, in the Southeastern Brazilian population, the GSTT1, GSTM1 and CYP2E1 polymorphisms and putative risk factors are associated with an increased risk for gastric cancer.

METHODS: We conducted a study on 100 cases of gastric cancer (GC), 100 cases of chronic gastritis (CG), and 150 controls (C). Deletion of the GSTT1 and GSTM1 genes was assessed by multiplex PCR. CYP2E1/PstI genotyping was performed using a PCR-RFLP assay.

RESULTS: No relationship between GSTT1/GSTM1 deletion and the c1/c2 genotype of CYP2E1 was observed among the three groups. However, a significant difference between CG and C was observed, due to a greater number of GSTT1/GSTM1 positive genotypes in the CG group. The GSTT1 null genotype occurred more frequently in Negroid subjects, and the GSTM1 null genotype in Caucasians, while the GSTM1 positive genotype was observed mainly in individuals with chronic gastritis infected with H. pylori.

CONCLUSION: Our findings indicate that there is no obvious relationship between the GSTT1, GSTM1 and CYP2E1 polymorphisms and gastric cancer.

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