Zhu LX, Liu J, Xie YH, Kong YY, Ye Y, Wang CL, Li GD, Wang Y. Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells. World J Gastroenterol 2004; 10(20): 2972-2978
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Professor Yuan Wang, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Yueyang Road 320, Shanghai 200031, China. email@example.com
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Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells
Li-Xin Zhu, Jing Liu, You-Hua Xie, Yu-Ying Kong, Ye Ye, Chun-Lin Wang, Guang-Di Li, Yuan Wang
Li-Xin Zhu, Jing Liu, You-Hua Xie, Yu-Ying Kong, Ye Ye, Chun-Lin Wang, Guang-Di Li, Yuan Wang, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Author contributions: All authors contributed equally to the work.
Supported by the National High Technology Research and Development Program of China, No. 2001AA215171
Correspondence to: Professor Yuan Wang, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Yueyang Road 320, Shanghai 200031, China. firstname.lastname@example.org
Received: December 24, 2003 Revised: January 11, 2004 Accepted: February 13, 2004 Published online: October 15, 2004
AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis.
METHODS: A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO) cell line. Cell proliferation was assessed by 3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis.
RESULTS: Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line.
CONCLUSION: HCV E2 can induce apoptosis in cultured mammalian cells.