Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 1, 2004; 10(11): 1625-1629
Published online Jun 1, 2004. doi: 10.3748/wjg.v10.i11.1625
Gene expression profiles of hepatocytes treated with La (NO3) 3 of rare earth in rats
Hui Zhao, Wei-Dong Hao, Hou-En Xu, Lan-Qin Shang, You-Yong Lu
Hui Zhao, Wei-Dong Hao, Hou-En Xu, Lan-Qin Shang, Department of Toxicology, Peking University Health Science Center, Beijing 100083, China
You-Yong Lu, School of Oncology, Beijing Institute for Cancer Research, Peking University, Beijing 100034, China
Author contributions: All authors contributed equally to the work.
Supported by grant of Key Project of National Natural Science Foundation of China, No. 29890280-3
Correspondence to: Wei-Dong Hao, Department of Toxicology, Peking University Health Science Center, No 38 Xue Yuan Road, Beijing 100083, China.
Telephone: +86-10-82802352 Fax: +86-10-62015583
Received: August 1, 2002
Revised: August 4, 2002
Accepted: August 31, 2002
Published online: June 1, 2004

AIM: To compare the gene expression between La (NO3) 3-exposed and control rats in vivo.

METHODS: Rats were fed La (NO3) 3 once daily at a dose of 20 mg/kg for one month by gavage. Gene expression of hepatocytes was detected using mRNA differential display (DD) technique and cDNA microarray and compared between treated and control groups.

RESULTS: Six differentially expressed sequence tags were cloned by DD, of which five were up regulated and one was down regulated in treated rats. Two sequences were determined. One band was novel. The other shared 100% sequence homology with AU080263 Sugano mouse brain mncb Mus musculus cDNA clone MNCb-5435 5’. With DNA microarray, 136 differentially expressed genes were identified including 131 over-expressed genes and 5 under-expressed genes. Most of these differentially expressed genes were cell signal and transmission genes, genes associated with metabolism, protein translation and synthesis.

CONCLUSION: La (NO3) 3 could change the expression levels of some kinds of genes. Further analysis of the differentially expressed genes would be helpful for understanding the wide biological effect spectrum of rare earth elements.

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