Purification of heat shock protein 70-associated tumor peptides and its antitumor immunity on hepatoma in mice

Figure 1 SDS-PAGE analysis of HSP70-tumor peptide com-plexes (silver staining). M: protein molecular weight marker, 1: protein eluted with 250-350 mmol/L NaCl buffer, after ADP-agarose chromatography (eluted with 0.5 mol/L NaCl buffer, PH7.5, containing 20 mmol/L Tris-acetate) and DEAE-ion exchange, 2-4: protein eluted with 250-350 mmol/L NaCl buffer, after ADP-agarose chromatography (eluted with 3 mmol ADP/L buffer, pH7.5, containing 20 mmol/L Tris-acetate) and DEAE-ion exchange.

Figure 2 Western blot identification of HSP70-tumor peptide complexes purified from HCaF cells. The notes are the same with Figure 1.

Figure 3 Immunoprotective effect of HSP70-associated pep-tides against tumor.

Figure 4 Protective effect of transferred immune spleen cells against repeated challenges with HCaF. —▲―▲ ×_× First, second, and third challenge controls. ●_● Transferred immune spleen cell group.