Construction of exogenous multiple epitopes of helper T lymphocytes and DNA immunization of its chimeric plasmid with HBV pre-S2/S gene

doi:10.3748/wjg.v10.i20.2979

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Oct 15, 2004 (publication date) through May 1, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Oct 15, 2004; 10(20): 2979-2983
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.2979

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Figure 1 Electrophoresis of ultimate product of MTE5 and its middle oligonucleotide fragments. Lane 1: Ultimate product of MTE5; Lanes 2 and 3: The middle oligonucleotide frag-ments of MTE5; Lane M: DNA marker.

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Figure 2 RFLP analysis of recombinant plasmid pUMTE5. Lane M: DNA marker; Lane 1: Splicing product of MTE5; Lanes 2 and 3: Plasmid pUC18 and recombinant plasmid pUMTE5 digested with restriction endonuclease Xba I; Lane 4: Recom-binant plasmid pUMTE5 digested with restriction endonu-cleases Xba I and Bam HI.

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Figure 3 DNA and amino acid sequences of MTE5.

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Figure 4 A: RFLP analysis of recombinant plasmid pcHB and chimeric plasimd pcHB-MTEX. Lane 1: Recombinant plasmid pUHB digested with restriction endonucleases Hind III and Eco RI; Lanes 2 and 3: Plasmid pcDNA3 and recombinant plas-mid pcHB digested with restriction endonuclease Hind III; Lane 4: Recombinant plasmid pcHB digested with restriction endonucleases Hind III and Eco RI; Lane M: DNA marker. B: RFLP analysis of the chimeric plasmid pcHB-MTEX. Lane M: DNA marker; Lane 1: MTE5; Lanes 2 and 3: Recombinant plas-mid pUHB and pUHB-MTEX digested with restriction endo-nuclease Eco RI; Lane 4: Recombinant plasmid pUHB-MTEX digested with restriction endonuclease Xba I; Lane 5: recom-binant plasmid pUHB-MTEX digested with restriction endo-nucleases Xba I and Eco RI; Lane 6: Recombinant plasmid pUHB-MTEX digested with restriction endonucleases Hind III and Eco RI; Lanes 7 and 8: Recombinant plasmid pcHB and pcHB-MTEX digested with restriction endonuclease Eco RI; Lane 9: Recombinant plasmid pcHB-MTEX digested with restriction endonucleases Hind III and Eco RI.

Citation: Gao WJ, Peng XM, Xie DY, Xie QF, Gao ZL, Yao JL. Construction of exogenous multiple epitopes of helper T lymphocytes and DNA immunization of its chimeric plasmid with HBV pre-S2/S gene. World J Gastroenterol 2004; 10(20): 2979-2983
URL: https://www.wjgnet.com/1007-9327/full/v10/i20/2979.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i20.2979