Construction of exogenous multiple epitopes of helper T lymphocytes and DNA immunization of its chimeric plasmid with HBV pre-S2/S gene

Figure 4 A: RFLP analysis of recombinant plasmid pcHB and chimeric plasimd pcHB-MTEX. Lane 1: Recombinant plasmid pUHB digested with restriction endonucleases Hind III and Eco RI; Lanes 2 and 3: Plasmid pcDNA3 and recombinant plas-mid pcHB digested with restriction endonuclease Hind III; Lane 4: Recombinant plasmid pcHB digested with restriction endonucleases Hind III and Eco RI; Lane M: DNA marker. B: RFLP analysis of the chimeric plasmid pcHB-MTEX. Lane M: DNA marker; Lane 1: MTE5; Lanes 2 and 3: Recombinant plas-mid pUHB and pUHB-MTEX digested with restriction endo-nuclease Eco RI; Lane 4: Recombinant plasmid pUHB-MTEX digested with restriction endonuclease Xba I; Lane 5: recom-binant plasmid pUHB-MTEX digested with restriction endo-nucleases Xba I and Eco RI; Lane 6: Recombinant plasmid pUHB-MTEX digested with restriction endonucleases Hind III and Eco RI; Lanes 7 and 8: Recombinant plasmid pcHB and pcHB-MTEX digested with restriction endonuclease Eco RI; Lane 9: Recombinant plasmid pcHB-MTEX digested with restriction endonucleases Hind III and Eco RI.