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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 14, 2017; 23(2): 224-231
Published online Jan 14, 2017. doi: 10.3748/wjg.v23.i2.224
Published online Jan 14, 2017. doi: 10.3748/wjg.v23.i2.224
Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells
Hong-Bo Shi, Hong-Lin Shi, Feng Ren, Zhong-Ping Duan, Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China
Jin-Li Lou, Clinical Laboratory Center, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China
Yu Chen, Zhong-Ping Duan, Artificial Liver Center, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China
Author contributions: Shi HB and Lou JL contributed equally to this work; Shi HB and Shi HL carried out most of the experiments; Ren F purified the strain and performed PCR; Chen Y analyzed the pattern of restriction enzyme digestion; Shi HB drafted the manuscript, and Lou JL analyzed the experimental data; Duan ZP and Lou JL conceived and supervised the study; Duan ZP was involved in editing the manuscript; all authors read and approved the final manuscript.
Supported by National Natural Science Foundation of China , No. 81300349 and No. 81270532 ; the Beijing Natural Science Foundation , No. 7144216 ; the Beijing Nova Program , No. Z131107000413016 ; the Project of Science and Technology Activities of Preferred Overseas Personnel of Beijing (2014) ; the Project of Cultivation of High Level Medical Technical Personnel in the Health System of Beijing , No. 2014-3-090 and No. 2013-3-071 ; Beijing Municipal Institute of public medical research development and reform pilot project , No. 2016-2 .
Conflict-of-interest statement: Shi HB, Lou JL, Shi HL, Ren F, Chen Y and Duan ZP are employees of Beijing Youan Hospital, Capital Medical University. The authors declare no conflicts of interest.
Data sharing statement: Technical appendices, statistical codes and datasets are available from the corresponding author at duan2517@163.com. Participants provided informed consent for data sharing. No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Zhong-Ping Duan, MD, PhD, Professor, Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, 8 Xitoutiao, Youwai Street, Fengtai District, Beijing 100069, China. duan2517@163.com
Telephone: +86-10-63291007 Fax: +86-10-63295258
Received: August 2, 2016
Peer-review started: August 3, 2016
First decision: September 21, 2016
Revised: October 5, 2016
Accepted: November 2, 2016
Article in press: November 2, 2016
Published online: January 14, 2017
Peer-review started: August 3, 2016
First decision: September 21, 2016
Revised: October 5, 2016
Accepted: November 2, 2016
Article in press: November 2, 2016
Published online: January 14, 2017
Core Tip
Core tip: Until now, there have been few specific mouse lines that allowed recombination for tracing hepatic mesothelial cells or hepatic stellate cells. Here, we describe a rapid and reliable strategy for construct preparation using a bacterial artificial chromosome. This study prepared a Gpm6a/ReelinGFPCreERT2 construct for the first time, which is the first step for the preparation of a Gpm6aGFPCreERT2 or ReelinGFPCreERT2 mouse line.